Supplemental table 1. Primer sequences used for RT-PCR, qRT-PCR, and ChIP assays

Nov 02, 2014

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Supplemental table 1. Primer sequences used for RT-PCR, qRT-PCR, and ChIP assays. RT-PCR, qRT-PCR. Primer sequences used for ChIP assay.

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Supplemental table 1. Primer sequences used for RT-PCR, qRT-PCR, and ChIP assays

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Supplemental table 1. Primer sequences used for RT-PCR, qRT-PCR, and ChIP assays RT-PCR, qRT-PCR Primer sequences used for ChIP assay

Supplementary Table 1 Sequences of the primer used for q-PCR analysis

Supplementary Table 1 Sequences of the primer used for q-PCR analysis hGAPDH forward, 5’-AGCAATGCCTCCTGCACCACCAAC-3’ hGAPDH reverse, 5’-CCGGAGGGGCCATCCACAGTCT-3’ hPARP1 forward, 5’-TCTACCCCCTGGAGATTGACT-3’ hPARP1 reverse, 5’-GGTCGATCTCATACTCCACCAT-3’

252 views • 7 slides

PCR Primer Design

PCR Primer Design. http://www.modares.ac.ir/elearning/mnaderi/Genetic%20Engineering%20course%20II/images/2a.jpg. Definition. PCR primer design is the creation of short nucleotide sequences for use in amplifying a specific region of DNA. Examples. PCR primers are designed to:

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PCR and Primer Design

PCR. PCR. PCR. PCR. Exercise 1. A) Estimate the number of sequences that have unwanted

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Supplemental table 1 : List of primers used for Me-DIP coupled Q-PCR.

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RT-PCR lab

RT-PCR lab. You have a cell…is a certain gene on (by “on,” we mean active and producing mRNA?)? If a certain gene is on when the cell divides, the gene might produce a protein that causes cell division…. Central Dogma:. DNA has genes and is in nucleus

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Supplementary Table 1. PCR primer sequences used in this study

Supplementary Table 1. PCR primer sequences used in this study. Supplementary Table References 1. Xu RH, Peck RM, Li DS, et al. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods 2005;2: 185-190.

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RT-PCR for NMDAR 1

RT-PCR for NMDAR 1. Base Pairs. V1. IHEC.

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Table S1. Primers used for semiquantitative RT-PCR

Table S1. Primers used for semiquantitative RT-PCR. 4. 4. 10. 10. 3. 3. 10. 10. 93.4. 96.6. 2. 2. 10. 10. 1. 1. 10. 10. 0. 0. 10. 10. 0. 1. 2. 3. 4. 0. 1. 2. 3. 4. 10. 10. 10. 10. 10. 10. 10. 10. 10. 10.

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Real-time RT-PCR

[Figure 3. Expression analysis of alternative transcripts in various human tissues including different brain tissues.]. 8%. Human. Real-time RT-PCR. Gorilla. Orangutan. LTR33. Gibbon. Old World monkeys. New World monkeys. Prosimians. Million years.

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Supplemental Table 1. Primer sequences for cloning and real-time RT-PCR of miRNA precursors.

Supplemental Table 1. Supplemental Table 1. Primer sequences for cloning and real-time RT-PCR of miRNA precursors. Supplemental Table 2. Supplemental Table 2. Primer sequences for detection of mature miRNAs. Supplemental Table 3.

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Supplementary table 1: Primers used for PCR amplification

Primer name. Sequence 5`-3`. Accession Number. ChiF. ACCAAGCTACTCGCAAGAGG. CD485880. ChiR. CGGAAGCGCAGTAAGATGA. GluF. CATTGATATGACCTTGATCG. CD486342. GluR. GTGAGATATCCCTTGGATTG. PR1F. TGCCCAAGACTCACAACAAG. CK988133.1 (Patil et al. 2005). PR1R. GGCCTTCTCATTAACCCACA. Hist3F.

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PCR AND PRIMER DESIGN

PCR AND PRIMER DESIGN. BIOL1220 – SYNTHETIC BIOLOGY. PRIMER DESIGN. WHAT IS PCR?. Repeated heated and cooling. What is polymerase? What is chain reaction?. Developed by Kary Mullis. Few DNA strands to millions of copies.

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Table S1. Primers and Probes Used in RT-PCR and qMSP

Supplementary Information. Table S1. Primers and Probes Used in RT-PCR and qMSP. Probe. Gene. Forward Primer. Reverse Primer. RT-PCR. GAPDH. GATATCTGGGTCATCGCTTC. TGAGTTTCCGTGAGGCTGAG. EMX2. GATATCTGGGTCATCGCTTC. TGAGTTTCCGTGAGGCTGAG. GAPDH EMX2. qPCR. Hs99999905_m1 Hs00244574_m1.

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Primer sequences for RT-PCR, ChIP assay and gene cloning analyses in human cells

Additional File 5:. Primer sequences for RT-PCR, ChIP assay and gene cloning analyses in human cells. Genes. Forward Primer. Reverse Primer. MnSOD. 5’-CTTCAGCCTGCACTGAAGTT-3’. 5’-CTGAAGGTAGTAAGCGTGCT-3’. p21 WAF1. 5′-CGACTGTGATGCGCTAATGG-3′. 5′-CCGTTTTCGACCCTGAGAG-3′. GAPDH.

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Supplementary Table 1 Sequences of quantative RT-PCR primers

Supplementary Table 1 Sequences of quantative RT-PCR primers. Supplementary Table 2 Body weight, plasma parameters and blood pressure of E-DNI  B mice. Supplementary Table 3 Body weight and plasma parameters of E-DNI  B mice. Supplementary Figure 1. Lung. Aorta. Macrophages.

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PCR & PCR Primer design

PCR & PCR Primer design. Talk by Joy Scaria. Applications of PCR. Molecular cloning. DNA sequencing. Archeology. Forensics. Amplification of unknown sequences. Clinical pathology. Genetic diagnosis. Characterizing unknown mutations. Fingerprinting/population analysis.

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RT-PCR

60. 50. 40. 30. RT-PCR. Fold change. 20. microarray. 10. 0. -10. -20. S101 vs. WT. A5 vs. WT. MP (AT1G19850). MP (AT1G19850). SAP (AT5G35770). AGP12 (AT3G520). ML1 (AT4G21750). AHA1 (AT2G18960). SEUSS (AT1G43850). WOX 13 (AT4G355550). SCARECROW-LIKE 3 (AT1G50420).

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Table S2. Sequences of primers used for RT-PCR

Supplementary Materials. Table S1. Features of patients with acute myocardial infarction or unstable angina and control subjects. AMI: acute myocardial infarction. * ANOVA test after the values were logarithm-transformed, # Chi-square test. ACEI: angiotensin convert enzyme inhibitors.

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KESRRP001 RT- PCR report

2009. 07. 07. KESRRP001 RT- PCR report. 1. Sample check. C sample. C -> control 1.5ug loading. 2009. 07. 07. KESRRP001 RT- PCR report. 2. Primer sequence. 2009. 07. 07. KESRRP001 RT- PCR report. 3. PCR Condition. 2009. 07. 07. KESRRP001 RT- PCR report.

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RT-PCR

RT-PCR. Detection of Specific RNAs. Annealing of Downstream Primer to RNA. Reverse Transcription With AMV Reverse Transcriptase. RNA Copied From 3’ to 5’ into cDNA. Amplification of cDNA by PCR.

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Supplemental Table 1. Primer sequences for cloning and real-time RT-PCR of miRNA precursors.

113 views • 10 slides

More Related

Supplemental table 1. Primer sequences used for RT-PCR, qRT-PCR, and ChIP assays

Supplemental table 1. Primer sequences used for RT-PCR, qRT-PCR, and ChIP assays

Presentation Transcript

Supplementary Table 1 Sequences of the primer used for q-PCR analysis

PCR Primer Design

PCR and Primer Design

Supplemental table 1 : List of primers used for Me-DIP coupled Q-PCR.

RT-PCR lab

Supplementary Table 1. PCR primer sequences used in this study

RT-PCR for NMDAR 1

Table S1. Primers used for semiquantitative RT-PCR

Real-time RT-PCR

Supplemental Table 1. Primer sequences for cloning and real-time RT-PCR of miRNA precursors.

Supplementary table 1: Primers used for PCR amplification

PCR AND PRIMER DESIGN

Table S1. Primers and Probes Used in RT-PCR and qMSP

Primer sequences for RT-PCR, ChIP assay and gene cloning analyses in human cells

Supplementary Table 1 Sequences of quantative RT-PCR primers

PCR &amp; PCR Primer design

RT-PCR

Table S2. Sequences of primers used for RT-PCR

KESRRP001 RT- PCR report

RT-PCR

Supplemental Table 1. Primer sequences for cloning and real-time RT-PCR of miRNA precursors.

PCR & PCR Primer design