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FOLIC ACID GRAFTING TO Ag NANOCLUSTERS-SILICA THIN FILMS

FOLIC ACID GRAFTING TO Ag NANOCLUSTERS-SILICA THIN FILMS. S. Ferraris , S. Perero, E. Vernè, L. Rimondini, E. Battistella and M. Ferraris. Cancer cells differ from heathy one for growth control, morphology, cell-to-cell interactions, membrane properties, cytoskeletal structure,

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FOLIC ACID GRAFTING TO Ag NANOCLUSTERS-SILICA THIN FILMS

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  1. FOLIC ACID GRAFTING TO Ag NANOCLUSTERS-SILICA THIN FILMS S. Ferraris, S. Perero, E. Vernè, L. Rimondini, E. Battistella and M. Ferraris

  2. Cancer cells differ from heathy one for • growth control, • morphology, • cell-to-cell interactions, • membrane properties, • cytoskeletal structure, • protein secretion, • vascularization • gene expression • … THE PROBLEM CANCER CELLS  RAPID DIVISION  FOLIC ACID NEED FOR DNA SYNTHESIS Cancer cells detection at the early developement stage.

  3. Characteristic SPR absorption FA FA FA Absorbance [a.u] Wavelength [nm] Ag Ag Ag Ag Ag AIM of THE WORK New SPR absorption Silver nanoclusters-silica composites thin films S. Ferraris, S. Perero, E. Vernè, E. Battistella, L. Rimondini; M. Ferraris “Surface functionalization of Ag-nanoclusters-silica composite films for biosensing accepted to Materials Chemistry and Physics

  4. SUBSTRATE SUBSTRATE Ar Ar SILVER NANOCLUSTERS-SILICA LAYER Ag SILICA THE SILVER NANOCLUSTERS-SILICA FILM

  5. 100 nm 100 nm SPR absorption SPR absorption THE SILVER NANOCLUSTERS-SILICA FILM AS DEPOSITED TT 450°C S. Ferraris, S. Perero, E. Vernè, E. Battistella, L. Rimondini; M. Ferraris “Surface functionalization of Ag-nanoclusters-silica composite films for biosensing accepted to Materials Chemistry and Physics

  6. Surface pre-treatment  cleaning and hydroxyls exposition OH OH OH Acetone and water washings Silanization Functional group = Amino-group 3-aminopropyltriethoxysilane (APTES) Folic Acid (FA) Grafting FA NH2 NH2 DCC FA FA FA Ag Ag Ag Ag Ag Ag Ag Ag Ag Si Si APTES APTES APTES THE GRAFTING PROCEDURE S. Ferraris, S. Perero, E. Vernè, E. Battistella, L. Rimondini; M. Ferraris “Surface functionalization of Ag-nanoclusters-silica composite films for biosensing accepted to Materials Chemistry and Physics

  7. a b NH2 XPS survey analysis XPS survey analysis NH2 Atomic % Atomic % Counts Element Washed Silanized Element Washed Silanized Counts O 47,6 40,3 O 57,5 46,6 C 28,2 35,5 C 17,3 28,1 Si 17,8 17,4 Si 22,3 18,4 Ag 6,3 4,4 Ag 3 2,4 N 0 2,4 N 0 4,5 Binding Energy [eV] Binding Energy [eV] SILANIZATION AS DEPOSITED TT 450°C S. Ferraris, S. Perero, E. Vernè, E. Battistella, L. Rimondini; M. Ferraris “Surface functionalization of Ag-nanoclusters-silica composite films for biosensing accepted to Materials Chemistry and Physics

  8. NH2 NH2 XPS survey analysis XPS survey analysis C-C C-C Atomic % Si Si Si NHCO Counts NHCO Atomic % Counts Element Washed Silanized FA+DCC NH2 NH2 NH Counts NHCO NHCO Element Washed Silanized FA+DCC O 57,5 46,6 37,4 Counts O 47,6 40,3 34,6 COOH C 17,3 28,1 41,4 C 28,2 35,5 38,9 Si 22,3 18,4 14,7 COOH Si 17,8 17,4 14,8 Ag 3 2,4 0,8 Ag 6,3 4,4 3,8 N 0 4,5 5,8 Binding Energy [eV] Ag Ag Ag Ag Ag N 0 2,4 7,9 Binding Energy [eV] Binding Energy [eV] Binding Energy [eV] FOLIC ACID GRAFTING AS DEPOSITED TT 450°C S. Ferraris, S. Perero, E. Vernè, E. Battistella, L. Rimondini; M. Ferraris “Surface functionalization of Ag-nanoclusters-silica composite films for biosensing accepted to Materials Chemistry and Physics

  9. FA FA FA REACTION WITH CROMOGENEOUS COMPOUND AND UV-MEASUREMENT OF ABSORBANCE Ag Ag Ag Ag Ag ELISA TEST (*) SECONDARY ANTIBODY (Rabbit polyclonal to Mouse IgG (HRP) ) PRIMARY ANTIBODY (FOLATE 8/33) (*) Eastern Piedmont University A. Avogadro S. Ferraris, S. Perero, E. Vernè, E. Battistella, L. Rimondini; M. Ferraris “Surface functionalization of Ag-nanoclusters-silica composite films for biosensing accepted to Materials Chemistry and Physics

  10. Folic acid grafted Ag-nanoclusters-silica thin films are able to selectively bind the proper antibody S. Ferraris, S. Perero, E. Vernè, E. Battistella, L. Rimondini; M. Ferraris “Surface functionalization of Ag-nanoclusters-silica composite films for biosensing accepted to Materials Chemistry and Physics

  11. CONCLUSIONS … • Silver nanoclusters-silica composite films have been effectively silanized with 3-aminopropyltriethoxysilane • Folic acid has been successfully grafted to silanized films via carbodiimide activation • The characteristic SPR absorption is maintained after functionalization • FA-functionalized films are able to selectively recognize the proper antibody

  12. … AND FUTURE DEVELOPMENTS • SPR absorption measurement in presence of the antibody will be performed in humid environment in order to evaluate the shift due to selective binding • Recognition experiment with marked healthy and cancerous cells will be performed

  13. Thanks!

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