1 / 42

結構生物學期末報告

結構生物學期末報告. 生技所 蕭孟昌 學號 M93360039. Structure of the DNA-binding domains from NFAT, Fos and Jun bound specifically to DNA. Science March 1998 VOL 392 P42-48 Lin Chen*, J. N. Mark Glover* † , Patrick G. Hogan ‡ , Anjana Rao ‡ & Stephen C. Harrison* †

merritt-koch
Download Presentation

結構生物學期末報告

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 結構生物學期末報告 生技所 蕭孟昌 學號 M93360039

  2. Structure of the DNA-bindingdomains from NFAT, Fos andJun bound specifically to DNA • Science March 1998 VOL 392 P42-48 • Lin Chen*, J. N. Mark Glover*†, Patrick G. Hogan‡, Anjana Rao‡ & Stephen C. Harrison*† • * Department of Molecular and Cellular Biology and †Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138, USA‡Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA • PDB ID :1A02

  3. WHAT QUESTION IS THIS PAPER WANT TO SOLVE • The interaction between NFAT (nuclear factor of activated T cells) and AP-1(Fos-Jun). • Explain the mechanism of combinatorial regulation of transcription.

  4. WHAT WE CAN DO IN THIS STUDY • What amino acid sequences are conserved and what are these sequences function? • What DNA sequences are conserved and what are these sequences function? • Try to find out other TF has similar mechanism to NFAT and AP-1.

  5. ANALYSIS OF NFAT

  6. CONSERVED REGION OF NFAT • TRP399. • SER429(與DNA之間有INTERANTION) • ASN451. • THR604(與DNA之間有INTERANTION) • PRO643(與AP-1之間有INTERACTION) • PRO677(與AP-1之間有INTERACTION)

  7. ANALYSIS OF AP-1

  8. CONSERVED REGION OF AP-1 • ALA150(與DNA之間有INTERACTION) • ARG159(與NFAT之間有INTERACTION) • GLU160 (與NFAT之間有INTERACTION) • SER279(與NFAT和DNA之間都有INTERACTION) • VAL294(Jun and Fos 的交錯點)

  9. ANALYSIS OF DNA SEQUENCE • DNA fragment that has the sequence of the distal antigen-receptor response element (ARRE2) from the murine IL-2 promoter23.

  10. 分析結果 • DNA sequence conserved region 生物上的功能性雖不若protein sequence 的顯著，但很明顯的和轉錄因子結合的DNA sequence仍具有高度的保留性。

  11. AP FAMILY • 命運法則 • 調控基因的能力是否相同？

  12. THANK YOU

More Related