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1 ICAR-DGR, Junagadh 2 Oilseeds Research Station, TNAU, Coimbatore

Enhancing oleic acid content using marker assisted introgression of fatty acid desaturase ( ahFAD2 ) alleles in peanut ( Arachis hypogaea L.).

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1 ICAR-DGR, Junagadh 2 Oilseeds Research Station, TNAU, Coimbatore

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  1. Enhancing oleic acid content using marker assisted introgression of fatty acid desaturase (ahFAD2) alleles in peanut (ArachishypogaeaL.) Sandip K.Bera1*, Jignesh H.Kamdar1, Swati V. Kasundra1, Pitabas Dash1, Anil K.Maurya1, Mital D. Jasani1, Ajay B. Chandrashekar1, N. Manivannan2, R. P. Vasanthi3, K. L. Dobariya4 Manish K. Pandey5, Pasupuleti Janila5, T. Radhakrishnan1, Rajeev K. Varshney5 1ICAR-DGR, Junagadh 2 Oilseeds Research Station, TNAU, Coimbatore 3 Reg. Agrii. Res. Stn., ANGRAU,Tirupati 4 Main Oilseeds Research Station, JAU, Junagadh 5 ICRISAT, Hyderabad

  2. ~50% oil Fodder ~25% Protein

  3. ~25 m ha in SAT region ~ 45.2 m tons Major producers

  4. Fatty acids composition of peanut Oil

  5. Desirable fatty acid

  6. Undesirable fatty acids

  7. Normal Groundnut High Oleic mutant Oleic acid Oleic acid Oleoyl-PC desaturase Oleoyl-PC desaturase X Less/ no Linoleic acid Linoleic acid

  8. Oleoyl - PC desaturase activity

  9. High oleic acid content in peanut mutant

  10. AhFAD2A AhFAD2B

  11. Details of allele specific markers used

  12. Allele-Specific primers for selection of FAD2A and FAD2B mutations

  13. 193bp band – Amplify in wild/ A genome substitution / B genome Insertion • Do not amplify in double mutations • 203bp band – Amplify in A genome substitution/ Double mutation • 195bp band – Amplify in B genome insertion/ Double mutation

  14. ICGV 5141 & ICGV 6100 SunOleic 95R F1 F2 BC1F1 BC2F1 BC3F1 BC3F5 BC1F5 BC2F5 F5 A total of 204 lines were developed in early generation

  15. Selection of high Oleate lines by Allele specific markers SO 95R Mutation in A Genome (Substitution) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 SO 95R Mutation in B Genome (Insertion) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23

  16. Oleic, linoleic & palmitic acids in ILs & parents

  17. Oleic/ linoleic acid ratio in ILs & parents

  18. Oil % in ILs and Parents

  19. Multi location testing of high oleate line Oleic acid content in parents and IL

  20. Oil and protein content in parents and IL

  21. Fatty acid profile of various cooking oil

  22. High oleic peanut club Argentina USA Australia Very soon India will join to this elite club

  23. We acknowledge Thanks

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