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Veterinary Medical Center

CLONING DOGS AND CATS: WHERE DO WE STAND? Margaret V. Root Kustritz, DVM, PhD, DACT University of Minnesota. U NIVERSITY OF M INNESOTA. SFT-ACT 2008. Veterinary Medical Center. U NIVERSITY OF M INNESOTA. SFT-ACT 2008. Veterinary Medical Center. CLONING - Nuclear transfer

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Veterinary Medical Center

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  1. CLONING DOGS AND CATS: WHERE DO WE STAND? • Margaret V. Root Kustritz, DVM, PhD, DACT University of Minnesota UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  2. UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  3. CLONING - Nuclear transfer Nucleus from a single cell from the donor is transferred into an oocyte that has had its DNA removed, the new oocyte fertilized and matured in vitro, and the subsequent embryo transferred into a recipient dam at the morula or blastocyst stage. UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  4. CLONING - Nuclear transfer - Any donor cell can be used (skin biopsy from the ear) UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  5. After transfer of DNA, the single cell is stimulated to begin division IVM = in vitro maturation Immature oocyte released Low E2 / P4 environment Cumulus cells must be present UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  6. UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  7. SUCCESS RATES - Cats IVF and IVM - 50% development to the blastocyst stage Cats have been cloned (“Copy Cat”) UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  8. UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  9. SUCCESS RATES - Dogs IVF and IVM - 8-37% development to the blastocyst stage One lab has produced cloned dogs. UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  10. “Snuppy” = Seoul National University + puppy UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  11. CONCERNS - Disease of cloned animals - Early senescence? - Increased stillbirths - Birth defects - Embryo wastage - Cat return rate 1.0% - Dog return rate 0.1% UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  12. CONCERNS - Disease of cloned animals - Early senescence? - Increased stillbirths - Birth defects - Embryo wastage - Cat return rate 1.0% - Dog return rate 0.1% UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  13. CONCERNS - Embryo wastage* - Cat return rate 1.0% - Dog return rate 0.1% - Cattle return rate 11.0% - Mouse return rate 5.0% - Goat and sheep return rate 3.0% * Using adult cell for donor nucleus UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  14. CONCERNS - Expense - Public perception “Little Nicky” UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  15. CONCERNS - Expense - Public perception “Rainbow” and her clone, “Copy Cat” UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

  16. COMMERCIAL AVAILABILITY - Projected cost = $50,000 / animal - Genetic Savings and Clone Website refers you to ViaGen – “Note that ViaGen has no plans to provide commercial dog or cat cloning services.” UNIVERSITY OF MINNESOTA SFT-ACT 2008 Veterinary Medical Center

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