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RNA 和 RNA 组学 （ RNomics) 研究进展

RNA 和 RNA 组学 （ RNomics) 研究进展. Reference: An expanding universe of noncoding RNA. 2002. Science. 296:1260-1263 ( www.sciencemag.org ) Glimpses of a tiny RNA world. 2001. Science. 294:797-799. Non-coding RNAs: the architects of eukaryotic complexity. 2001.

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RNA 和 RNA 组学 （ RNomics) 研究进展

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  1. RNA和RNA组学（RNomics)研究进展

  2. Reference: An expanding universe of noncoding RNA. 2002. Science. 296:1260-1263 (www.sciencemag.org) Glimpses of a tiny RNA world. 2001. Science. 294:797-799. Non-coding RNAs: the architects of eukaryotic complexity. 2001. EMBO Reports. 2(11):986-991. (embo-reports.oupjournals.org)

  3. DNA 基因组学 RNA RNA组学 蛋白质 蛋白质组学

  4. 人： 30,000 果蝇和线虫：12,000-14,000 酿酒酵母： 6,200 假单孢菌： 5,500 人和鼠的蛋白质编码基因99%是共同的。 人个体间单倍体基因组的碱基差异300万个，其中1万个（0.3%）出现在蛋白质编码基因中，且绝大多数存在于第3字母。98%转录物是非编码蛋白RNA。 2倍 2倍 蛋白质编码基因数量

  5. RNA的种类及主要功能 Ribosomal RNAs (rRNAs): translation Transfer RNAs (tRNAs): translation Messenger RNAs (mRNAs): protein template Noncoding RNAs(ncRNAs): various types and functions

  6. mRNA、tRNA、rRNA的功能

  7. 多聚核糖体的电镜和示意图

  8. 原核生物核糖体的大亚基和小亚基

  9. 原核和真核生物的核糖体

  10. 原核和真核生物核糖体的装配

  11. 原核和真核生物的转录和翻译过程

  12. 核糖体的解聚与重新装配

  13. 蛋白质的翻译过程

  14. 翻译过程中的转位

  15. 翻译过程的终止

  16. 基因的三联密码表

  17. 三联密码子按简并性排列

  18. tRNA中参与氨酰tRNA合成酶 的识别因子

  19. 酵母丙氨酸tRNA的一级结构

  20. tRNA的氨酰化反应

  21. tRNA的氨酰化反应

  22. The Complete Atomic Structure of the Large Ribosomal Subunit at 2.4 Å Resolution Science. 2000. 289:905-919. Nenad Ban,1* Poul Nissen,1* Jeffrey Hansen,1 Peter B. Moore,1, 2 Thomas A. Steitz1, 2, 3 1 Department of Molecular Biophysics & Biochemistry, and 2 Department of Chemistry, Yale University, and 3 Howard Hughes Medical Institute, New Haven, CT 06520-8114, USA. *   These two authors contributed equally to this work.

  23. 核糖体的电子密度图

  24. 核糖体大亚基旋转花冠图

  25. 23S rRNA的二级结构

  26. 核糖体大亚基RNA的四级和二级结构

  27. 核糖体大亚基RNA的四级和二级结构（续）

  28. 23S rRNA的保守和扩展序列

  29. 大亚基核糖体蛋白质骨架结构

  30. 核糖体大亚基表面的蛋白质

  31. 蛋白质延伸入RNA和 多域的相互作用

  32. The Structural Basis of Ribosome Activity in Peptide Bond Synthesis Science. 2000. 289:920-930. Poul Nissen,1* Jeffrey Hansen,1* Nenad Ban,1* Peter B. Moore,1, 2 Thomas A. Steitz1, 2, 3 1 Department of Molecular Biophysics and Biochemistry and 2 Department of Chemistry, Yale University, and 3 Howard Hughes Medical Institute, New Haven, CT 06520-8114, USA. *   These authors contributed equally to this work.

  33. 肽基转移酶底物和类似物的化学结构

  34. 氧：红 磷：黄 氮：兰 碳：绿 底物：灰 底物类似物的相位电子密度图

  35. A-和P-位点结合的组合模型

  36. 23S\5S rRNA、蛋白质和组合CCA的填空模型

  37. 域5活性位点区的立体图

  38. The closest approach of polypeptides to the peptidyl transferase active site marked by the Yarus inhibitor, CCdA-p-Puro

  39. Conserved nucleotides in the peptidyl transferase region with bound CCdA-p-Puro

  40. 肽基转移酶活性位点的催化装置

  41. 多肽的出口通道

  42. Crystal Structure of the Ribosome at 5.5 Å Resolution Science. 2001. 292:883-896. Marat M. Yusupov,1* Gulnara Zh. Yusupova,1* Albion Baucom,1 Kate Lieberman,1 Thomas N. Earnest,2 J. H. D. Cate,3 Harry F. Noller1 1 Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, CA 95064, USA. 2 Berkeley Center for Structural Biology, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. 3 Whitehead Institute, Cambridge, MA 01242, USA.

  43. Electron density of tRNAMetf bound to the P site of the 70S ribosome, at 5.5 Å resolution

  44. 70S核糖体的结构

  45. Secondary and tertiary structures of 16S, 23S, and 5S rRNAs

  46. Conformational differences between rRNAs in 70S ribosomes and 30S and 50S subunits

  47. 亚基间桥

  48. 亚基间桥（续）

  49. tRNA-ribosome interactions

  50. Two views of the A-tRNA anticodon stem loop bound to its codon in the 30S subunit A site

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