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Arabidopsis ecotypes infected with Fusarium oxysporum (WT) and a fosnf1 mutant. Cape Verde Col-0 EST. H 2 O. WT. fosnf1. GFP-labeled F. oxysporum colonizing the root xylem of Arabidopsis. II Hyphal transfer. I
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Arabidopsis ecotypes infected with Fusarium oxysporum (WT) and a fosnf1 mutant Cape Verde Col-0 EST H2O WT fosnf1
GFP-labeled F. oxysporum colonizing the root xylem of Arabidopsis
II Hyphal transfer I Cocultivation + selection + Mono-conidial isolation III Agrobacterium cells + Fungal spores (1x105) IV Single spore culture Transformants (10-100 / plate) Phenotype characterization Agrobacterium tumefaciens-Mediated Transformation of Fungi
OCCURRENCE OF AVR-Pita IN THE USA FIELD ISOLATES OF M. grisea B C F G H A D E BM5-11 BM1-24 75A11 CA205 CA305 75A49 75A10 LO1-4 LO3-8 82A1 85M2 A598 A264 A119 75A1 A347 85L2 85A7 74T3 49D 793 D1 S1 Distribution pattern of the AVR-Pita gene family among rice isolates of Magnaporthe grisea Probe Fs
PCR of marker genes PCR of marker genes DNA extraction from pure cultures of plant pathogens DNA extraction from diseased plant or cultured isolate Sequencing/Genotyping of marker genes Pathogen Database Database searching Database building Known phenotypes of pathogen isolates Risk assessment & implementation of control/eradication measures Schematic diagram of the plant pathogen database