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Cholangiocarcinoma in PSC: Opportunities within a Study Group

Dr. Steve Pereira presents a study on the characterization of serum proteins in biliary tract cancer, PSC, and IAC using 2D-DIGE and LC-MS/MS. The study identifies differentially expressed serum proteins and proposes clinical follow-up for patients with suspicious biliary strictures.

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Cholangiocarcinoma in PSC: Opportunities within a Study Group

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  1. International PSC Study Group Meeting, Oslo, 14-15 Jun 2010Cholangiocarcinoma in PSC: Opportunities within a study group Dr Steve Pereira Institute of Hepatology UCL Medical School & University College Hospital

  2. Characterisation of serum proteins in biliary tract cancer, PSC and IAC using 2D-DIGE and LC-MS/MS Neomal S Sandanayake1,2,3, John Sinclair3, Fausto Andreola2, Michael H Chapman1,2, Stephane Camuzeaux3, George J Webster1,2, Ian D Norton4 , Ross C Smith5, John F Timms3, Stephen P Pereira1,2 1Department of Gastroenterology and Hepatology, University College London Hospital; 2Institute of Hepatology, University College London; 3Institute for Women's Health, University College London; 4Department of Gastroenterology, 5Department of Surgery, Royal North Shore Hospital, University of Sydney, Australia Conclusions: Differentially expressed serum proteins were identified in BTC compared to PSC, IAC and healthy controls using serum immuno-affinity depletion, 2DDIGE and LC-MS/MS.

  3. Proposal Suspicious biliary strictures Imaging Bile / brushings Blood Tissue Biobank Clinical follow for > 1 yr in patients without proven malignant diagnosis Malignancy Benign disease

  4. TRANSBIL: TRANSlational research in BILiary tract cancer + Proposal Patients: >18 yrs Benign or suspected or proven malignant PB disease Written informed consent Samples: Biliary brushings and bile - in storage buffer (provided) Blood - NIH SOPs - inverted 5 times, 1 hour clot at room temp, centrifuged 2200rpm at 4°C, serum aliquoted into 1.5ml cryovials, -80° C Tissue samples -snap-frozen in liquid N2 and stored at -80°C - FFPE

  5. Pre-clinical samples 202,000 women - annual phlebotomy 270 individuals developed pancreatic cancer 54 individuals developed biliary tract cancer Resource UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) serum bank at UCL

  6. Cholangiocarcinoma in PSCOpportunities for trials? • Surgery / OLT • Stents • Radiotherapy / Brachytherapy • Chemoradiation • Chemotherapy • PDT

  7. April 6 2010

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