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Figure 1S. Clinical specimen: A/Massachusetts/12/2009: 31(AAT) = resistant S31N Pyrosequencing result: T CTC GTC A TT GCA GCA AAT ATC ATT GGG ATC. (A). Residue: L26 V27 A30 N31 G34.
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Figure 1S Clinical specimen: A/Massachusetts/12/2009: 31(AAT) = resistant S31N Pyrosequencing result: T CTCGTCATT GCA GCAAAT ATC ATT GGG ATC (A) Residue: L26 V27 A30 N31 G34 Triple reassortant swine isolate: A/Ohio/01/2007: wild type sequence at all 5 positions: sensitive Pyrosequencing result: T CTCATT GAT GCC GCAAGC ATC ATT GGG ATT (B) Residue: L26 I27 A30 S31 G34
Virus isolate: A/Wisconsin/53/2009: wild type sequence at all 5 positions: sensitive Pyrosequencing result: T CTCGTCATT GCA GCAAGT ATC ATT GGG ATC (C) Residue: L26 V27 30 S31 G34
Figure 2S Clinical specimen: A/Tennessee/08/2009 Pyrosequencing result: G GAT AAC TGG C Amino acid 295: AAC= sensitive N295 (A) Triple reassortant swine isolate: A/Illinois/09/2007 Pyrosequencing result: A GAC AAT TGG C Amino acid 295: AAT= sensitive N295 (B) Clinical specimen:A/Washington/272009 Pyrosequencing result: GAC at position 199 Amino acid 199: GAC= sensitive D199 (C) (K)
Virus isolate: A/North Dakota/04/2009 Pyrosequencing Result: T GTG TTT GTCATA AGG GAA C Amino acids 1116, 117, and 119, respectively: GTC= wild type V116, ATA= wild type I117, and GAA= wild type E119 (D) Amino acid 136: CAA= wild type Q136 Clinical specimen: A/New York/72/2009 Pyrosequencing result: ACT CAA GGG GCC (E) Amino acids 150, 151: AAA= wild type K150, GAC= wild type D151 Clinical specimen: A/Tennessee/08/2009 Pyrosequencing result: C ATT AAAGAC A (F)