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LexA Hybrid

LexA Hybrid. Gal4AD Hybrid. -His. 1. CD4c C394R/V395D. Gal4AD. 2. CD4c C394R/V395D. Lck. 3. CD4c C394R/V395D. Siva-1. 4. CD4c C397R. Gal4AD. 5. CD4c C397R. Lck. 6. CD4c C397R. Siva-1.

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LexA Hybrid

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  1. LexA Hybrid Gal4AD Hybrid -His 1 CD4c C394R/V395D Gal4AD 2 CD4c C394R/V395D Lck 3 CD4c C394R/V395D Siva-1 4 CD4c C397R Gal4AD 5 CD4c C397R Lck 6 CD4c C397R Siva-1 Figure legend: L40 expressing CD4c C394R/V395D or CD4c C397R mutants fused to LexA, and Siva-1or Lck fused to Gal4AD or Gal4AD alone was analyzed for histidine auxotrophy. Both mutants are able to interact with Siva-1, demonstrating that the two cysteine residues C394 and C397 are not critical for the interaction of CD4c with Siva-1. Figure S1

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