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myc- b 1. FLAG hsp90 [530-724]. FLAG hsp90 [1-309]. FLAG hsp90 [1-456]. FLAG hsp90 [1-530]. IP: anti-FLAG WB: anti- b 1. b 1. b 1. 100. 55.9. IP: anti-FLAG WB: anti-FLAG. 38.3. kDa. Cell lysate WB: anti- b 1. 100. 55.9. Cell lysate WB: anti-FLAG. 38.3. kDa.
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myc-b1 FLAG hsp90 [530-724] FLAG hsp90 [1-309] FLAG hsp90 [1-456] FLAG hsp90 [1-530] IP: anti-FLAG WB: anti-b1 b1 b1 100 55.9 IP: anti-FLAG WB: anti-FLAG 38.3 kDa Cell lysate WB: anti-b1 100 55.9 Cell lysate WB: anti-FLAG 38.3 kDa Fig. S1. COS cells were co-transfected with the cDNAs encoding myc-tagged full-length β1 and various deletion mutants of hsp90. Immuno- precipitation (IP) was performed using a FLAG antibody and precipitates were tested for the presence of β1. Blots are representative of experiments repeated at least twice with identical results. Numbers identify the relative positions in the amino acid sequence; retained sequences are bracketed. MOLPHARM/2005/012682Fig S1