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The Role of the Microenvironment In the Pathogenesis of CLL Thomas J. Kipps, MD, PhD

The Role of the Microenvironment In the Pathogenesis of CLL Thomas J. Kipps, MD, PhD. …get by with a little help from my freinds. Viability of CLL Cells in Culture. w/ stroma. w/out stroma. Percent Viability. Days. “Nurse-like” Cells From the Blood of Patients with CLL.

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The Role of the Microenvironment In the Pathogenesis of CLL Thomas J. Kipps, MD, PhD

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  1. The Role of the Microenvironment In the Pathogenesis of CLL Thomas J. Kipps, MD, PhD

  2. …get by with a little help from my freinds

  3. Viability of CLL Cells in Culture w/ stroma w/out stroma Percent Viability Days

  4. “Nurse-like” Cells From the Blood of Patients with CLL

  5. Seed Blood Cells at 1 to 1.5 x 107/ml After 10 to 14 days, harvest non-adherent leukemia cells and put on another Flask for 3 hrs to eliminate contaminating adherent cells Seed on plate with or without nurse-like cells NLC (+) NLC (-)

  6. Survival of CLL Cells In vitro Dependent Upon Nurselike Cells with NLC without NLC Percent Viability Days in Culture

  7. CLL Cells Cultured Without NLC Experience Reduction in Mcl-1, PARP Cleavage, and Caspase Activation Survival In Culture 3 2 1 pre- culture PARP 3 with NLC day 3 w/o NLC day 3 1 T=0 uncleaved cleaved % viability with NLC Caspase 3 w/o NLC proform 2 active p17 Days after re-seeding Mcl-1 Bcl-2

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