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The Effects of Auxin on Seed to Seed Germination of Brassica rapa

Objective and Purpose. To find out if supplemental auxin has an effect on seed output and germination rates.Using substances in agriculture to promote seed output and germination. . AUXIN-Phytohormone . Auxin is characterized as both a hormone and a morphogen. (Friml, 2003; Campbell

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The Effects of Auxin on Seed to Seed Germination of Brassica rapa

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    1. The Effects of Auxin on Seed to Seed Germination of Brassica rapa Courtney Lee, Ashley Smith, Kandace Thornton, Andre’lyn Williams

    2. Objective and Purpose To find out if supplemental auxin has an effect on seed output and germination rates. Using substances in agriculture to promote seed output and germination.

    3. AUXIN-Phytohormone Auxin is characterized as both a hormone and a morphogen. (Friml, 2003; Campbell & Reece, 2005). The hormone auxin has an effect on the patterns and differentiation of plant tissues (Reinhardt, 2003). Auxin response factors (ARFs) are responsible for the transcriptions and mediate of the responses of the plant hormone (Ellis, 2005).

    4. Auxin on Embryogenesis An experiment designed by Philippar et al. (2006) concluded that the auxin-induced K+ channel gene Zmk1 is essential for embryo development and coleptile growth. Philippar et al. study suggested that auxin plays a crucial role in embrogenesis.

    5. Materials Fast Plant kit including Styrofoam cells, wicks, seeds, soil, and fertilizer pellets Auxin IAA Potassium Salt

    6. Methods Plant seeds according to instructions on Fast Plant Website Reduce cells to one sprout, maintain water level, and stake plants.

    7. Before Pollination

    8. Pollination Pollinate after 14 days for 4 days.

    9. After Pollination

    10. Obtaining Results Harvest after 20 days after pollination. Count number of seeds and allow seeds to germinated, count number of seeds germinated.

    11. Results

    12. Discussion (1. Show promotion) Auxin promotes germination rates and the output of Brassica rapa seeds; using auxin on crops could enhance the output. (2. Indifference) Auxin did not show noticeable effects on germination rates and output of Brassica rapa seeds; testing auxin on other crops might produce different results.

    13. Further Research Test auxin on other crops and plants, especially those used in agricultural economy. Test other substances, such as gibberellins, cytokinins, and ethylene, on crops and Brassica rapa.

    14. Literature Cited Campbell, N., & Reece, J. (2005). Exploring Life. In B. Wilbur (Ed.), Biology (7th ed., p. 16). San Francisco: Pearson Benjamin Cummings. Ellis, C., Nagpal, P., Young, J., Hagen, G., Guilfoyle, T., & Reed, J. (2005). AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana. Development, 132, 4563-4574. Retrieved February 21, 2006, from Cambridge Scientific Abstracts database. Friml, J. (2003). Auxin transport — shaping the plant. Plant Biology, 6(1), 7-12. Retrieved February 1, 2007, from Science Direct database. Philippar, K., Büchsenschütz, K., Edwards, D., Löffler, J., Lüthen, H., Kranz, E., et al. (2006). The auxin-induced K+ channel gene Zmk1 in maize functions in coleoptile growth and is required for embryo development. Plant Molecular Biology, 61(4), 757-768. Retrieved February 20, 2006, from Cambridge Scientific Abstracts database. Reinhardt, D. (2003). Vascular Patterning: More Than Just Auxin? Current Biology, 13(12), R485-R487.

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