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GAS CHROMATOGRAPHY

GAS CHROMATOGRAPHY. Mr. Maywan Hariono. One of chromatography technique that use a gas as a carrier (mobile phase) The separation is based on the volatility of sample Same with HPLC, GC is one of advance chromatography method and use Rt as a separation parameter. Phase??.

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GAS CHROMATOGRAPHY

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  1. GAS CHROMATOGRAPHY Mr. Maywan Hariono

  2. One of chromatography technique that use a gas as a carrier (mobile phase) • The separation is based on the volatility of sample • Same with HPLC, GC is one of advance chromatography method and use Rt as a separation parameter

  3. Phase?? • Stationary phase: octadecyl silane, octasilane, aminosilane • Mobile phase (gas) : He, N2, H2, Ar

  4. The principle and instrumentation of GC

  5. The volatile sample in the liquid matter is injected to the column. The temperature of column is setted in which sample can be vaporized. The gas is pumped and then it carries the sample (gas) through the column. The sample which has higher boiling point/melting point will be retained by column, in contrast the lower one will be carried through the column faster. Means the higher b.p/m.p wil be lower the Rt and vice versa Principles

  6. The information from GC machine will be translated to the graph by software Principles

  7. Komponen2 GC • syringe • Injection port • column • GC oven • detector • Carrier gas • recorder

  8. Syringe 5-10 microliter in volume GC Component

  9. b. Injection port GC Component

  10. c. Column GC Component

  11. Factors influence the GC separation performance i.Boiling point/melting point ii. Column temperature iii. Column length iv. The speed of gas stream v. Internal diameter of column

  12. GC APPLICATIONS 1. Pharmaceutical industry 2. Herbal medicine industry 3. Environmental analysis 4. Cosmetics analysis

  13. adavntages • Precise and accurate • Sensitive

  14. Disadvantages GC • Time consuming • Only volatile sample can be determined using GC iii. The sample is damaged after running, can not be recollected.

  15. Rujukan 1.David G Watson(2005). Pharmaceutical Analysis. Second edition. Pp: 236-265 2.http://www.en.wikipedia.org/wiki/Gas-chromatography.html 3.http://orgchem.colorado.edu/hndbksupport/GC.html

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