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Overview of Dried Blood Spots

Overview of Dried Blood Spots. Advantages of Dried Blood Spots (DBS). Easy collection Simple transportation Easy and compact storage Safer to handle Centralized laboratory can perform testing. What type of lancet should be used?.

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Overview of Dried Blood Spots

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  1. Overview of Dried Blood Spots

  2. Advantages of Dried Blood Spots (DBS) • Easy collection • Simple transportation • Easy and compact storage • Safer to handle • Centralized laboratory can perform testing

  3. What type of lancet should be used? • Incision device with a blade that cuts to a controlled, standardized depth. This provides better blood flow than a standard needle lancet. • Device should have a blade which permanently retracts after use. This prevents the lancet from being used a second time and protects those individuals handling lab waste.

  4. DBS collection cards • These cards are not ordinary blotting paper. • The paper is designed with specific characteristics related to absorbency and density. • This assures that you have a specific volume of blood within each printed circle (specific to the card).

  5. DBS can be collected from Vacutainers of blood (venous puncture) or from finger sticks.

  6. Conditions that affect DBS • Heat • DBS should not be exposed to high temperatures • Store DBS in refrigerator for short term (1-2 weeks) • Or in freezer for long term (months - years) • Humidity • After blood is spotted onto cards it should be completely dried • DBS must be packaged in airtight bags with desiccant • Accurate volume of blood collected onto the card

  7. Good Quality DBS

  8. Poor Quality DBS

  9. Poor Quality DBS

  10. As with other lab samples, good record keeping is important

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