Conclusions

1. The Effect of Cartilage Tissue Extraction of 13 Days-old Mouse Embryo on Differentiation of Bone Marrow Mesenchymal Stem Cells to Osteoblasts Ramezani M. 1 , Khanbabaee R. 2 , Hojati V. 1 1. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran 2. Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran Results Objectives Results At the first, mesenchymal stem cells were spindle shape. After affect of extraction during 21 days, osteoblast cells and calcium crystals were observed. in control grope, because of high potential differentiation of MSCs had spontaneous differentiation into osteoblasts. The groups of 10, 30 and 60 concentration showed significant differentiation increases than control group (0.05>p). Result of morphological and staining intensity of osteoblastic cells is provided below. Mesenchymal Stromal Progenitor/Stem Cells (MSCs) are a rare population of non-hematopoietic stromal and multipotent cells, present in the bone marrow and most connective tissues of the body, which during development are derived from cells with a mesenchymal origin. MSCs have two proprietary features: differentiation and self_renewal. Also they have high proliferative potential in the long term and are able to differentiation into mesenchymal tissues such as osteoblast, osteocyte, adipocyte , condrocyte and muscle. In vitro osteoblastic differentiation needs some factors, which creates condition similar to bone tissue microenvironment. In previous study, researchers used of inducer factors like beta_glycerophosphate and ascorbate for in vitro differentiation of MSCs into osteoblast. Shape-2 : (Differentiation of mesenchymal stem cells into osteoblasts after AR staining. A) Control group, B) 10% density, C) 30% D) 60%) shape-1: (Mesenchymal stem cell population) Methods Figure 1: (Result of osteoblastsic cell staining analyses) Conclusions Bone marrow mesenchymal stem cell isolated from adult Balb/C mouse femur and cultured with conventional method. Mesenchymal stem cells were purred by several passages. After seven subculture, they affected of 13 days of mouse embryo tissue extraction groups with density of 10% , 30% and 60%. They treated for 21 days in osteogenic culture and stained by Alizarin Red. Then, morphological and staining intensity of osteoblastic cells was studied. Effect of cartilaginous tissue extraction of 13 days-old mouse embryo on mesenchymal stem cells increased osteoblastic differentiation. Also osteoblastic differentiation is directly related to density of extract. The results show that osteogenic inducer like bone morphogenic protein-2(BMP-2), transforming growth factor(TGF), WNTs and other factors present in cartilaginous tissue extract. This study demonstrate that mesenchymal stem cell are capable to differentiate to osteoblast in vitro and can be use as a new inducing factor. References