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Overview: The generation of genetic mosaics affecting a splicing reporter in Drosophila. hsFLP; AcyGAL4 UAS-eGFP X UAS-cDNA ; Splicing reporter. cross strains and heat shock to induce FLP. hsFLP. FLP. AyGAL4. GAL4. yellow +. GAL4. FRT. FRT. UAS-eGFP. UAS-cDNA. eGFP.

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  1. Overview: The generation of genetic mosaics affecting a splicing reporter in Drosophila hsFLP; AcyGAL4 UAS-eGFP X UAS-cDNA ; Splicing reporter cross strains and heat shock to induce FLP hsFLP FLP AyGAL4 GAL4 yellow+ GAL4 FRT FRT UAS-eGFP UAS-cDNA eGFP splicing factor UAS UAS Splicing reporter LacZ AUG Stop normal cells Immunostain tissue elevated splicing factor GAL4 GAL4 FLP eGFP (green) marks GAL4 clones lacZ (red) reporter activated by splicing factor lacZ (red) reporter repressed by splicing factor Outcome: A system for observing splicing factor function in vivo Question answered: How do changes in factor activity affect splicing in a specific tissue?

  2. A B GFP Splicing reporter

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