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Cell Impedance Measurement System: Cell Culture and Interface Board Design

Cell Impedance Measurement System: Cell Culture and Interface Board Design. Emma Berry & Austin Grimes Dr. Nicole McFarlane, Khandaker Mamun , Geneva Doak YSP/RET Final Presentations July 18, 2013 Knoxville, Tennessee. Cell Impedance Measurement System. Cell Culturing Method.

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Cell Impedance Measurement System: Cell Culture and Interface Board Design

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  1. Cell Impedance Measurement System: Cell Culture and Interface Board Design Emma Berry & Austin Grimes Dr. Nicole McFarlane, KhandakerMamun, Geneva Doak YSP/RET Final Presentations July 18, 2013 Knoxville, Tennessee

  2. Cell Impedance Measurement System

  3. Cell Culturing Method

  4. Cell Captures

  5. Subculture

  6. Neutral Red

  7. Printed Circuit Boards(PCBs) • Printed Circuit Boards are circuits that are compacted into a small board. • Printed Circuit Boards are used to connect a device, or cells, to an interface like the lock in amplifier.

  8. Printed Circuit Boards(PCBs) • The circuit boards we have created have all been created in Eagle, a software that is specifically made for designing PCBs. • In Eagle, you must start off by making a schematic for the software to know what connects to what. 8 Pin Switch 10 Pin Sockets BNC Port

  9. Printed Circuit Boards(PCBs) • Once you are done with the schematic, you press a button that transfers you to the board layout. • The pieces are off to the side and you put them on to the board where you want them to go. • Although this may sound easy, it is like a complex puzzle. BNC Port 8 Pin Switch 10 Pin Socket

  10. Printed Circuit Boards(PCBs) • Once you have everything where you want it on the board, you add a silkscreen. • The silkscreen tells the board factory what you need to be printed on the board.

  11. Printed Circuit Boards(PCBs) • When we receive the board from the factory, we will need to solder any pieces onto the board that we need for our studies. BNC Port 10 Pin Socket 8 Pin Switch

  12. Printed Circuit Boards(PCBs) • Once we solder the pieces on to the board, it is ready to test with the cells. BNC Port 8 Pin Switch 10 Pin Socket

  13. Results

  14. Conclusion • Contributions successful • Future work

  15. Acknowledgements • We thank Dr. Chien-fei Chen, Dr. Nicole McFarlane, Khandaker Abdullah Al Mamun, and Geneva Doak for their guidance and encouragement during this project. We also thank CURENT for providing this program and experience for young scholars.

  16. References Freshney, R. Ian. Culture of Animal Cells: A Manual of Basic Technique. New York: Wiley, 2000. Hamilton, T.J.; Nelson, N.M.; Sander, D.; Abshire, Pamela, "A cell impedance sensor based on a silicon cochlea," Biomedical Circuits and Systems Conference, 2009. BioCAS 2009. IEEE , vol., no., pp.117,120, 26-28 Nov. 2009 Prakash, S.B.; Nelson, N.M.; Haas, A.M.; Jeng, V.; Abshire, Pamela; Urdaneta, M.; Smela, Elisabeth, "BioLabs-On-A-Chip: Monitoring Cells using CMOS Biosensors," Life Science Systems and Applications Workshop, 2006. IEEE/NLM , vol., no., pp.1,2, July 2006 Reeves, N.; Liu, Y.; Nelson, N.M.; Malhotra, S.; Loganathan, M.; Lauenstein, J.-M.; Chaiyupatumpa, J.; Smela, E.; Abshire, P.A., "Integrated MEMS structures and CMOS circuits for bioelectronic interface with single cells," Circuits and Systems, 2004. ISCAS '04. Proceedings of the 2004 International Symposium on , vol.3, no., pp.III,673-6 Vol.3, 23-26 May 2004 Urdaneta, M.; Christophersen, Marc; Smela, Elisabeth; Prakash, S.B.; Nelson, N.; Abshire, Pamela, "Cell Clinics Technology Platform for Cell-Based Sensing," Life Science Systems and Applications Workshop, 2006. IEEE/NLM , vol., no., pp.1,2, July 2006

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