Chapter 10 DNA Replication and Recombination

1. Chapter 10 DNA Replication and Recombination

2. Chapter Contents 10.1 DNA Is Reproduced by Semiconservative Replication 10.2 DNA Synthesis in Bacteria Involves Five Polymerases, as well as Other Enzymes 10.3 Many Complex Tasks Must Be Performed during DNA Replication 10.4 A Summary of DNA Replication in Prokaryotes 10.5 Replication in Prokaryotes Is Controlled by a Variety of Genes Continued

3. 10.6 Eukaryotic DNA Synthesis Is Similar to Synthesis in Prokaryotes, but More Complex 10.7 Telomeres Provide Structural Integrity at Chromosome Ends but are Problematic to Replicate 10.8 DNA Recombination, Like DNA Replication, Is Directed by Specific Enzymes 10.9 Gene Conversion Is a Consequence of DNA Recombination

4. Section 10.1: DNA Is Reproduced by Semiconservative Replication • The complementarity of DNA strands allows each strand to serve as a template for synthesis of the other. (Figure 10.1)

5. Section 10.1 • Three possible modes of DNA replication are possible: • conservative • semiconservative • dispersive (Figure 10.2)

6. Figure 11.2

7. Section 10.1 • The Meselson-Stahl experiment demonstrated that: • DNA replication is semiconservative • each new DNA molecule consists of one old strand and one newly synthesized strand (Figure 10.3 and Figure 10.4)

10. Section 10.1 • The Taylor-Woods-Hughes experiment demonstrated that DNA replication is semiconservative in eukaryotes (Figure 10.5)

14. Section 10.1 • DNA replication begins at the origin of replication and is bidirectional rather than unidirectional (Figure 10.6).

15. Section 10.1 • A replicon is the length of DNA that is replicated following one initiation event at a single origin.