190 likes | 303 Views
Tristan Ursell Group Meeting 10-22-2010. One or both of the following are true: - PG has a natural curvature that dictates cell width - MreB in vivo has a natural curvature that, via PG construction, dictates cell width . Project #1 Regrowth of the Cell Wall.
E N D
One or both of the following are true: - PG has a natural curvature that dictates cell width - MreBin vivo has a natural curvature that, via PG construction, dictates cell width Project #1Regrowth of the Cell Wall
Grow cells in penicillin or vancomycin and treat with lysozyme. Regrowth of the Cell Wall Errington Nature 2009
Regrowth of the Cell Wall Errington Nature 2009
Regrowth of the Cell Wall Berndl EPL 1990 Wurtis PRE 1997 Seifert PRA 1991
Regrowth of the Cell Wall What proteins cause it to curve? How is the binding symmetry broken? Tseng J BiolChem 2005
In what morphologies does the cell wall regrow in L-forms? (Phase, cytosolic GFP, FM 464 membrane labeling) Where is the DNA in ‘dividing’ L-forms? (DAPI labeling) Is mreB necessary for cell wall regrowth? (A2 depolymerization) Where is mreB in L-form bacteria? (mreB-GFP) How do L-forms propagate? (murE inducible strain?) Regrowth of the Cell Wall
Membrane-Mediated Protein Interactions How strong is orientational ordering? To what degree can shape encode specificity? What are the kinetics of formation of different complexes? What are the different phases that exist?
Membrane-Mediated Protein Interactions Mason PRL 2009
Membrane-Mediated Protein Interactions Rothemund Nature 2006