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DNA Replication. Mr. Mah Living Environment. DNA (de-oxy-ribo-nucleic acid) a nucleic acid that stores and transmits the genetic information from one generation to the next. Where in the cell is DNA found?. People knew that DNA was made up of:. Adenine (A) Guanine (G) Cytosine (C)
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DNA Replication Mr. Mah Living Environment
DNA(de-oxy-ribo-nucleic acid)a nucleic acid that stores and transmits the genetic information from one generation to the next
People knew that DNA was made up of: • Adenine (A) • Guanine (G) • Cytosine (C) • Thymine (T)
Erwin Chargaff • Discovered that in any sample of DNA, the amount of A equals the amount of T and the amount of C equals the amount of G. • This suggested that A binds with T and that C binds with G.
Rosalind Franklin’sX-Ray Crystallography • Took x-ray photographs of crystallized DNA • Discovered that the shape of DNA must be a helix, because of the x-ray pattern • Died of cancer (too many x-rays?)
Watson and Crick • Credited for discovering the structure of DNA in 1953 • Used Chargaff and Franklin’s discoveries along with toy models to discover the structure
NUCLEOTIDES Made up of: • A phosphate group • 5 carbon sugar • Nitrogenous Base (nitrogen containing)
PURINES • Adenine (A) • Guanine (G)
PYRIMIDINES • Cytosine (C) • Thymine (T)
Base Pairing in DNA A-T C-G
Write the complementary DNA strand for each of these DNA strands: • DNA • GAATTACA • CCAATTAG • ATAGACAG • CCAGTACA • DNA • CTTAATGT • GGTTAATC • TATCTGTC • GGTCATGT
Each of the 96 columns represents one DNA sequence. A different colored dye attached to each of the four nucleotides.
Replication The act of making an exact copy of DNA
Semi-Conservative Replication Each strand of the original molecule acts as a template for the synthesis of a new complementary DNA molecule
With semi-conservative replication, each new strand is ½ new and ½ old
RNA(ribo-nucleic acid)a nucleic acid that is a messenger between DNA and the ribosomes
Differences between DNA and RNA • Different sugars (DNA=deoxyribose) (RNA=ribose) • RNA is single stranded (1 side) DNA is double stranded (2sides) • RNA has “U” (uracil) DNA has “T” (thymine)
Always remember… RNAlovesU
Write the RNA strand for each of these DNA strands: • ½ Strand of DNA • GAATTACA • CCAATTAG • ATAGACAG • CCAGTACA • RNA • CUUAAUGU • GGUUAAUC • UAUCUGUC • GGUCAUGU
Transcription The process by which DNA is copied into mRNA
mRNA Messenger RNA, is the copy of the DNA that goes from the nucleus to the ribosome
tRNA Transfer RNA, matches up with mRNA and has an amino acid on its top
CODON Each combination of 3 nucleotides on a strand of mRNA
ANTICODON Each combination of 3 nucleotides on a tRNA
Translation The decoding of a mRNA message into proteins
rRNA Ribosomal RNA, glues together all of the amino acids into a polypeptide chain