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MCF7. MDA- MB-231. siP1. f. Relative hoxd4 expression. pri-miR-10b. 1.5. 1.2. siP2. 0.9. 0.6. *. d. *. *. 0.3. miR- 10a. Cont. siP2. siP1. 0. hoxd4. Cont. siAgo1. siAgo4. siAgo2. siAgo3. siDicer. β - actin. miR-10a. miR-10a. e. a. miR-10a. sinc34. sinc35.
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MCF7 MDA- MB-231 siP1 f Relative hoxd4 expression pri-miR-10b 1.5 1.2 siP2 0.9 0.6 * d * * 0.3 miR- 10a Cont siP2 siP1 0 hoxd4 Cont siAgo1 siAgo4 siAgo2 siAgo3 siDicer β-actin miR-10a miR-10a e a miR-10a sinc34 sinc35 Relative expression of hoxb/d4 sinc36 Cont 8 Cont siD1 siD2 miR-10a miR-10b 6 hoxd4 β-actin 4 sinc34 sinc35 sinc36 * Cont 2 b ** ** * 0 nc-hoxd4-34 nc-hoxd4-35 MDA- MB-231 MCF7 nc-hoxd4-36 β-actin hoxd4 hoxb4 c siP2 siP1