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Ectomycorrhizal dynamics of white fir stands at Teakettle

Ectomycorrhizal dynamics of white fir stands at Teakettle. A.D. Izzo and T.D. Bruns Plant and Microbial Biology University of California, Berkeley. Questions. • what is the community structure of ectomycorrhizal fungi associated with white fir?

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Ectomycorrhizal dynamics of white fir stands at Teakettle

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  1. Ectomycorrhizal dynamics of white fir stands at Teakettle A.D. Izzo and T.D. Bruns Plant and Microbial Biology University of California, Berkeley

  2. Questions • what is the community structure of ectomycorrhizal fungi associated with white fir? • how does species richness and diversity change following fire/thinning? • what is the role of the spore bank in these scenarios? • how does the spore bank respond to heat/ash?

  3. Sampling 0 5 10 20 White fir stand

  4. ITS1F ITS4 ID of root tips by RFLP matches Techniques: RFLP matching separate morphotypes freeze dry, weigh Species ID match PCR RFLP PCR RFLP DNA sequence (ITS) TaxonomicID (Phylum, “Family”, Genus, RFLP taxon)

  5. ECM biomass highly variable

  6. BK AB Basidio18 Thelephoroid2 Piloderma Piloderma2 Russuloid1 Russulaceae Basidiomycete Russula Agaricales Cortinarius Cortinariaceae Cortinarioid1 Pezizales Otideaceae Wilcoxina Wilcoxina1 Ascomycete Cencococcum Cencococcum3 RFLP-taxon

  7. Note minimal temporal similarity

  8. Comparison of treatments Minimal overlap of dominant RFLP-taxa

  9. Family-level comparison TK99 TK01/99 (2 yrs)

  10. ECM “islands of dominance” 3 1 2

  11. Additional studies Spatial bioassays - soils from all 54 plots pre-treatment - ABCO, PIJE, ABCO + PIJE Heat and Ash bioassays Pooled soil from 7-8 mature white fir stands across Teakettle C, 40, 55, 70 deg C, ash ABCO, PIJE

  12. Acknowledgements Molecular analysis Josephine Agbowo Collectors 1999/2001 Martin Bidartondo Ryan Bowman Francisco Camacho Lisa Grubisha Erik Lilleskov Bioassay setup Kari Cheng Funding USDA, PMB dept

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