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TEM of natural samples Whole-mounts - Roscoff October 2000-April 2001

TEM of natural samples Whole-mounts - Roscoff October 2000-April 2001 - Helgoland March and April 2001 - Blanes September 2000, April 2001 Thin-sections - Blanes September 2000 December 2000,

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TEM of natural samples Whole-mounts - Roscoff October 2000-April 2001

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  1. TEM of natural samples Whole-mounts - Roscoff October 2000-April 2001 - Helgoland March and April 2001 - Blanes September 2000, April 2001 Thin-sections - Blanes September 2000 December 2000, March 2001 April 2001

  2. Priority on TEM of cultures Results of examined natural samples Whole-mounts must improve quality Helgoland March 2001- bacteria, diatom, scales from Mamiellales and Chrysochromulina, Pelagococcus envelope?, broken coccoliths Roscoff March 2001-few cells, bacteria, Bathycoccus prasinos, choanoflagellate

  3. Priority on TEM of cultures Results of examined natural samples Thin-sections fixation OK, need more material Blanes September 2000-not filtered, cyanobacteria, B. prasinos, prymnesiophycean December 2000 - accident March 2001 - wrong decission

  4. Blanes Cyanophytes Prymnesiophyte Bathycoccus prasinos

  5. Helgoland March 2001

  6. Oslofjord Samples prepared October serial dilution culture, whole-mounts, thin-sections December serial dilution culture, whole-mounts, thin-sections January serial dilution culture, whole-mounts, thin-sections February Whole-mounts March serial dilution culture, hole-mounts, thin-sections

  7. Results from October 2000 Whole-mounts -Chrysochromulina apheles, C. Scutellum, cf. Dicrateria, Phaeocystis sp.Emiliania huxleyii -Micromonas pusilla, Mamiella gilva, -coccoids without scales, unknown with scales, Paraphysomonas sp. Thin-sections Cyanobacteria, M. Pusilla, Nephroselmis sp. , heterotrophic flagellates

  8. Serial dilution cultures from October Prymnesiophytes Chrysochromulina apheles, C. scutellum, Phaeocystis sp., Cryptomonads cf Hemilselmis, green cryptomonad 3-5 microns,, Prasinophytes M.pusilla, Nephroselmis sp. Pyramimonas sp. Cyanobacteria Red Serial dilutions from December and January almost empty. Too early for the March cultures

  9. Oslofjord October 2000 Cell with virus Pyramimonas sp. Cyanobacteria Unknown pico

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