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Loricrin

Supplementary 2.

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Loricrin

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  1. Supplementary 2. A) Western Blot analysis of HBP1, p63 and Loricrin in whole cell extracts from NHEK cells after the indicated time following differentiation (H, hours; D, days). Vinculin is used as internal control. B) ChIP analysis of p63 binding to the HBP1 promoter in NHEK. NF-Y. a transcription factor bound to the HBP1 promoter, was used as a positive control. C) Western Blot analysis of HBP1 after RNAi of HBP1 in primary human keratinocytes. All bands except one are decreased with the HBP1 siRNA with respect to the scramble siRNA control. B C A Si HBP1 Si Ctl 13 D 72h 24h 48h 7 D 8 D 6 D Ctl Vinculin Vinculin Input NF-Y p63 100 Ctl 70 Growing 55 HBP1 HBP1 Differentiated HBP1 promoter 35 27 p63 Loricrin Borrelli et al., Suppl. 2

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