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Investigation into Design of Micro Flow Cytometer Data Collection System

Investigation into Design of Micro Flow Cytometer Data Collection System. Sam Weiers. Nastaran Hashemi , Pouya Asrar. Outline. Motivation and objectives Approach, methods Current Status of work/current results Work in progress, planned work and timeline. Motivation/Objectives.

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Investigation into Design of Micro Flow Cytometer Data Collection System

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  1. Investigation into Design of Micro Flow Cytometer Data Collection System Sam Weiers NastaranHashemi, PouyaAsrar

  2. Outline • Motivation and objectives • Approach, methods • Current Status of work/current results • Work in progress, planned work and timeline

  3. Motivation/Objectives • Motivation • To create a micro flow cytometer that will analyze marine algae in UUVs • Objectives • Design and construct an electrical system • Run multiple electronic components • Collect and save data

  4. Approach/Methods • First Task: Create a program for data acquisition • Tools: • LabVIEW 2011 • NI USB-6351 DAQ Device • Excel 2010 (For Graphing)

  5. Approach/Methods

  6. Approach/Methods • Designed and Created Circuits for: • 1 PMT • 3 MPPCs

  7. Approach/Methods • Employ the micro channel into the system

  8. Approach/Methods • Employ the micro channel into the system

  9. Current Status/Results • DAQ program operational and stable • All MPPC and PMT circuits working • Can only collect data from two sources

  10. Future Work Planned • Add light filters to the system • Restrict wavelengths entering PMTs and MPPCs • Collect data from all four positions • Use living organisms instead of beads

  11. Citations • N. Hashemi, J.S. Erickson, J.P. Golden, F.S. Ligler, “Optofluidic Characterization of Marine Algae using a Microflow Cytometer”, Biomicrofluidics, 5, 032009 (2011) • PouyaAsrar. Micro Channel Design and Construction.

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