1 / 5

XMRV Protein Expression in Purified Activated T and B Lymphocytes from CFS Patients

See using a flow cytometry assay expression of 3 goat poly clonal antibodies to purified viral proteins in both T and B cells but not in T cells from a normal donor ..

nevin
Download Presentation

XMRV Protein Expression in Purified Activated T and B Lymphocytes from CFS Patients

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. See using a flow cytometry assay expression of 3 goat poly clonal antibodies to purified viral proteins in both T and B cells but not in T cells from a normal donor .. We have shown expression of XMRV proteins but next wanted to ask if that reflected the presence of infectious and transmissable virus 3 goat polyclonal antibodies to purified viral proteins XMRV Protein Expression in Purified Activated T and B Lymphocytes from CFS Patients Rat α-MuLV p30 Gag mAb

  2. Prostate cancer cell line Transmission of XMRV from Activated PBMCs of CFS Patients to the Human Prostate Cancer Cell Line LNCaP

  3. Using a technique known as spinoculation we transmitted virus from plasma and confirmed infectious particles by TEM next slide Cell Free Transmission of XMRV from CFS Patients Plasma to LNCap 21 positive of 25 (84%)

  4. Early and late buds type C morphology Mature extracellular viral particles with condensed centrally located nucleoid surrounded by an outer membrane separated by an electron lucent area Having detected the presence of XMRV viral proteins and particles in the PBMC of CFS patients, we next asked if we could detect an immune response to XMRV in CFS patients Infectious whole virus budding from the cell membrane Transmission Electron Micrograph of C-type Retrovirus Particles Transmitted from CFS patient T-Cells to LNCaP Infectious whole virus budding from the cell membrane

  5. Antibodies in CFS Patients Plasma to XMRV Env BaF3-ER BaF3-ER-SFFV Env BaF3-ER-SFFV Env BaF3-ER WB: αSFFV Env mAb BaF3-ER BaF3-ER-SFFV Env SFFV Env

More Related