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Instrumental Analysis, Laboratory Oral Presentation

Instrumental Analysis, Laboratory Oral Presentation. Nikko Bacalzo Douglas Dy Jeremy Ly France Tan. Determination of Anions in Toothpaste by Ion Chromatography . Instrument Used: Ion Chromatography. FLUORIDE. http://toxicfreekidsblog.org/2011/07/toxic-toothpaste /

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Instrumental Analysis, Laboratory Oral Presentation

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  1. Instrumental Analysis, LaboratoryOral Presentation Nikko Bacalzo Douglas Dy Jeremy Ly France Tan

  2. Determination of Anions in Toothpaste by Ion Chromatography Instrument Used: Ion Chromatography

  3. FLUORIDE http://toxicfreekidsblog.org/2011/07/toxic-toothpaste/ http://www.webmd.com/oral-health/image-collection-oral-health#phototake_rm_photo_of_tooth_illustration.jpg

  4. http://www.skepticnorth.com/2010/10/fluoridiated-water-debate-returns-nothing-new-is-said/http://www.skepticnorth.com/2010/10/fluoridiated-water-debate-returns-nothing-new-is-said/ http://www.fluoridation.com/teeth.htm

  5. ION CHROMATOGRAPHY

  6. ION CHROMATOGRAPHY http://www.separations.us.tosohbioscience.com/ServiceSupport/TechSupport/ResourceCenter/PrinciplesofChromatography/IonExchange

  7. ION CHROMATOGRAPHY Injection valve Readout Filter Column Mobile phase/ solvent reservoir Detector Pump Excess/ waste

  8. Data and Results

  9. Data and Results Fluoride calibration curve

  10. Data and Results Chloride calibration curve

  11. Data and Results

  12. Data and Results

  13. Conclusion • Four toothpaste brands were analyzed for their fluoride and chloride contents (Sensodyne, Colgate, Close-Up and Hapee). • Standards were run in the ion chromatograph, and the retention times and the concentration-peak area relationships of each were drawn from the data. • Out of which only Sensodyne and Hapee showed quantifiable amounts of chloride in the toothpaste formulation. • Calculation yielded concentrations of 0.00923% and 0.016% chloride in both toothpastes, respectively. • Other samples had no sufficiently quantifiable amounts of both anions, suspected to be consequence of matrix effect.

  14. Recommendations • Use other MP: carbonate/bicarbonate buffer, KOH • Use gradient elution • Standard addition instead of calibration curve • Homogenization of samples • Analyze other anions (MFP, benzoate, sulfate, phosphate) • Method validation

  15. References • Takahara, Y, et al. Photooxidation of organic compounds in a solution containing hydrogen peroxide and TiO2particles under visible light. Journal of Applied Chemistry. Vol.35, 793-797.<http://www.springerlink.com/content/t866330021785302/> • Shimadzu Corporation. The UV-2401PC: A Superbly Engineered Instrument.Accessed on May 21, 2012. <http://www.pa.ibf.cnr.it/web/pdf/12.pdf>

  16. Thank you for listening

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