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DMF-Modified. FKBP Digest Peptides: ProZap C 18. Fig. S4. Conventional. 2. 6. RT = 2.3 min. RT = 2.2 min. 5. 1.5. RT = 1.7 min. 4. RT = 1.8 min. Deuterium Level. 1. 3. 2. 0.5. 1. [74-80][M+H] +. [26-36][M+2H] 2+. 0. 0. 0. 30. 60. 90. 120. 0. 30. 60. 90. 120. 7.
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DMF-Modified FKBP Digest Peptides: ProZap C18 Fig. S4 Conventional 2 6 RT = 2.3 min RT = 2.2 min 5 1.5 RT = 1.7 min 4 RT = 1.8 min Deuterium Level 1 3 2 0.5 1 [74-80][M+H]+ [26-36][M+2H]2+ 0 0 0 30 60 90 120 0 30 60 90 120 7 2.5 RT = 0.9 min RT = 0.8 min 6 2 5 RT = 1.7 min Deuterium Level 1.5 4 RT = 1.8 min 3 1 2 0.5 1 [37-49][M+2H]2+ [55-59][M+H]+ 0 0 0 30 60 90 120 0 30 60 90 120 Time (min) Average 18% Higher D Level with DMF Modifier (38 Common Fragments)