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Sequencing Using DNA dependant DNA polymerase

*. dATP. dATP. dCTP. ddCTP. DNA template. dTTP. dATP. ddCTP. dGTP. dGTP. *. *. *. dCTP. dTTP. dATP. Denature & anneal labelled primer. Sequencing Using DNA dependant DNA polymerase. Initiation & elongation commences. ddCTP. *. ddCTP. DNA template. ddCTP.

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Sequencing Using DNA dependant DNA polymerase

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  1. * dATP dATP dCTP ddCTP DNA template dTTP dATP ddCTP dGTP dGTP * * * dCTP dTTP dATP Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase Initiation & elongation commences ddCTP

  2. * ddCTP DNA template ddCTP Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dTTP dATP dGTP dGTP * dTTP dATP * *

  3. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase Elongation terminated by dideoxyCTP dATP dATP ddCTP dCTP dTTP dATP dGTP ddCTP * dTTP dATP * * ddCTP

  4. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase Elongation continues for remaining molecules dATP dATP ddCTP dCTP dTTP dATP dGTP ddCTP * dTTP dATP * * ddCTP

  5. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dTTP dATP dGTP ddCTP * dTTP * * ddCTP

  6. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dATP dGTP ddCTP * dTTP * * ddCTP

  7. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dATP dGTP ddCTP * dTTP * * ddCTP

  8. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation dATP dATP ddCTP dCTP dATP dGTP ddCTP * * * ddCTP

  9. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation terminated by dideoxyCTP dATP dATP ddCTP dCTP dATP dGTP * ddCTP * * ddCTP

  10. * DNA template Denature & anneal labelled primer Sequencing Using DNA dependant DNA polymerase elongation terminated by dideoxyCTP dATP dATP dCTP dATP dGTP * ddCTP * ddCTP * ddCTP

  11. Sequencing Using DNA dependant DNA polymerase • Multiple products of different length terminated by A dideoxyCTP • Theses molecules represent all the positiions within the complementary sequence where a “C” occurs • Four separate reactions each terminated by a different dideoxy molecule would represent all bases • Ordering these molecules by size allows us to determine the template sequence * ddCTP * ddCTP * ddCTP * ddCTP * ddCTP * * ddCTP ddCTP

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