Topic 2: Cells

1. Topic 2: Cells

2. IB Objectives • 2.1 Cell Theory • 2.1.1 –Outline the cell theory • 2.1.2-Discuss the evidence for the cell theory • 2.1.3-State the unicellular organisms carry out all the functions of life • 2.1.4-Compare the relative sizes of molecules, cell membrane thickness, viruses, bacteria, organelles and cells, using the appropriate SI units • 2.1.5-Calculate the linear magnification of drawings and the actual size of specimens in images of known magnification • 2.1.6-Explain the importance of the surface area to volume ratio as a factor limiting cell size • 2.1.7-State the multicellular organisms show emergent properties • 2.1.8-Explain that cells in multicellular organisms differentiate to carry out specialized functions by expressing some of their genes but not other • 2.1.9-State that stem cells retain the capacity to divide and have the ability to differentiate along different pathways • 2.1.10-Outline one therapeutic use of stem cells

3. Cell Theory • Many scientists have contributed in developing the three main principles of this theory • All organisms are composed of one or more cells • Cells are the smallest units of life • All cells come from pre-existing cells

4. Evidence to back up #1 • Robert Hooke: 1665 • First described cells by observing cork with a microscope built by himself • Antonie van Leeuwenhoek: 1668 • First observed the living cells and referred to them as “animalcules” (little animals) • Mathias Schleiden: 1838 • Stated that plants are made of “independent, separate beings” called cells • Theodor Schwann: 1839 • Made a similar statement as Schleiden about animals

5. Evidence to backup #2 • The second principle continues to gain support today, as we have not been able to find any living entity that is not made of at least one cell

6. Evidence to backup #3 • Louis Pasteur: 1860 • He sterilized chicken broth by boiling to show that living organisms would not “spontaneously” reappear. • Only after exposing the broth to preexisting cells was life able to re-establish itself in the broth

7. Functions of Life • Metabolism • Growth • Reproduction • Response • Homeostasis • Nutrition

8. Functions of Life • All these functions are tied together to produce a functioning living unit • Metabolism includes all the chemical reactions that occur within an organism • Growth may be limited but is always evident in one way or another • Reproduction involves heredity molecules that can be passed to offspring • Homeostasis refers to maintaining a constant internal environment. • Nutrition is all about providing a source of compounds with many chemical bonds which can be broken to provide the organism with the energy and the nutrients necessary to maintain life

9. Cells and Sizes • Cells are made up of a, but all are microscopically number of different subunits • These subunits are often of a particular size, but all are microscopically small. • In most cases, microscopes with high magnification and resolution are needed to observe cells and especially their subunits • Resolution refers to the clarity of a viewed objects

10. Cells and Sizes • Light microscope-uses light, which passes through the living or dead specimens, to form an image. • Stains may used to improve viewing of parts • Electron microscope-provides us with the greatest magnification (over 100 00X) and resolution. • These use electrons passing through a specimen to form an image

11. Cells and Sizes • Level of Organization and size • Cells (most cells are up to 100 µm) • Organelles (most up to 10 µm) • Bacteria (most up to 1µm) • Viruses (most are 100 nm) • Membranes (most up to 10 nm) • Molecules (most up to 1 nm)

12. Cells and Sizes • If you want to calculate the actual size of a specimen seen with a microscope, you need to know the diameter of the microscope’s field of vision • This may be calculated with a special micrometer or with a simple ruler on a light microscope. • The size of specimen can then be calculated in the field

13. Cells and Sizes • The size of the specimen can then be calculated in the field • Drawing or photographs are often enlarged. • To calculate the magnification, you need this formula: • magnification = size of image divided by size of specimen • Scale bars are often used with a micrograph or drawing so that actual size can be determined

14. Calculating linear magnification of drawings or photos • A scale bar is a short line, usually drawn on an electron micrograph, to allow you to determine the actual magnification of the photograph

15. Calculating magnification • 1. measure scale bar carefully with a metric ruler, in mm. • 2. convert the mm into the same units as the scale bar (μm or nm) • 3. divide this by the number given on the scale bar (μm or nm)

16. Converting metric units

17. Example: • 15 mm = 15000 μm (change to same units as microscope photo) • 15000 μm ÷ 5 = 3000 • Magnification = 3000x Bar = 5 μm (measure bar with a metric ruler = 15 mm)

18. If the magnification is stated, you can calculate the actual size • 1. measure the diameter of the cell • 2. divide by the magnification • 3. convert to a commonly used unit (nm or μm)

19. Example:Magnification = 750x • 41 ÷ 750 = 0.055 mm • 0.055 mm = 55 μm • Diameter of the cell is 55 μm Diameter of cell is 41 mm (measure with metric ruler)

20. Practice: • p. 96, a and b (cilia) • p. 98 b (bacterium) • p. 105 fig 6.12 (ER)

21. Practice answers: • p. 96, a and b (cilia) 7mm = 1 μm = 7000x magnification (convert mm into μm) • p. 98 b (bacterium) 19mm = 19000 μm (conversion) 19000/0.5 = 38000x magnification

22. p. 105 fig 6.12 (ER) 9mm: convert to nm = 9,000,000 Divided by 200 = 45000x

24. Limiting Cell Size • The surface to volume ratio • In the cell, the rate of heat and waste production and rate of resource consumption are functions of (depend on) its volume • Most of the chemical reactions occur in the interior of the cell and its size affects the rate of thee reactions • The surface of the cell, the membrane, controls what materials move in and out of the cell • Cells with more surface area per unit volume are able to move materials in and out of the cell, for each unit volume of the cell

25. Limiting Cell Size • As the width of an object such as a cell increases, the surface area also increases but at a much slower rate than the volume • This is shown by the following table in which you can see that the volume increases by a factor calculated by cubing the radius; at the same time, the surface increases by a factor calculated by squaring the radius

26. Limiting Cell Size • This means that a large cell has relatively less surface area to bring in needed materials and to rid the cell of waste, than a small cell. • Because of this, cells are limited as to the size they can attain and still be able to carry out the functions of life. • Sphere formulas: • Surface area = (four)(pi)(radius squared) = 4πr2 • Volume = (four-thirds)(pi)(radius cubed) = 4/3πr3

27. Limiting Cell Size • This means large animals do not have larger cells they just have more cells • Cells that are larger in size have modifications that allow them to function efficiently • Shape changes-such as spherical to long and thin • Infolding and outfolding

28. Cell Reproduction and differentiation • One of the functions that many cells retain is the ability to reproduce themselves. • In multicellular organisms, this allows the possibility of growth. • It also allows for the replacement of damaged or dead cells • Multicellular organisms like ourselves usually start out as a single cell after some type of sexual reproduction

29. Cell Reproduction and differentiation • Multicellular organisms like ourselves usually start out as a single cell after some type of sexual reproduction • This single cell has the ability to reproduce at a very rapid rate, and the resulting cells then go through a differentiation process to produce all the required cell types that are necessary for the well-being of the organism

30. Cell Reproduction and differentiation • Some cells have a greatly, or even completely diminished ability to reproduce once they become specialized • Examples is muscle cells and nerve cells

31. Stem Cells • There is a population of cells within organisms that retain their ability to divide and differentiate into various cell types—Stem cells • Plant cells—found inside the meristematic tissue-near root and stem tips • Gardeners-take cuttings of this area and use them to produce new plants • Pluripotent or embryonic stem cells—cells in mammals which retain the ability to form any type of cell in an organism and can even form a complete organism

32. Stem Cells • When stem cells divide to form a specific type of tissue, they also produce some cells that remain as stem cells—this allows for the continual production of a particular type of tissue. • Possibility to treat certain human diseases • But a problem discovered early in research was that stem cells cannot be distinguished by their appearance only by their behavior.

33. Stem Cell Research and Treatments • Grow embryonic stem cells in culture so they can be used to differentiate cells lost due to injury and disease—This involves therapeutic cloning. • Parkinson’s disease and Alzheimer's disease—caused by a loss in brain cells • Certain forms of diabetes-depleted cells in pancreas • Still Experimental

34. Stem Cell Research and Treatments • Type of currently successful treatments • There is also tissue specific stem cells—these reside in certain tissue types and can only produces new cells of that particular tissue. • Example-blood stem cells—replace the damage bone marrow of some leukemia patients

35. Stem Cell Research and Treatments • There are important ethical issues involved in stem cell research. Especially controversial is the use of embryonic or pluripotent stem cells. • This is because these cells come from embryos often obtained from laboratories carrying out in-vitro fertilization (IVF). • To gather cells invloves death of the embryos and opponents argue that this represents the taking of a human life. • On the other hand, it is argued this research could result in the significant reduction of human suffering and is, therefore, totally acceptable