Haploid embryonic stem cells

1. Haploid embryonic stem cells Reporter：XueBinghua Date：2011.12.24

2. Derivation of haploid embryonic stem cells from mouse embryos Martin Leeb & Anton Wutz Nature 7 September 2011

3. Results: 1、Deriving haploid mouse ES cells

4. B6CBAF1 132 activated oocytes 30 blastocysts (22%) 27 ES cell lines(93%) LIF+2i[SU5402+PD184352(inhibitor of MAPK and GSK3)]

5. Requirements for deriving aploid mES cells 1、Lif+KOSR+DMEM2、Lif+FBS+DMEM+Feeder

6. Results: 2、DNA content

8. Results: 3、Morphology and Karyotype

10. Results: 4、Comparative genomichybridization

12. g CBA control, black; HAP-1, red; HAP-2, green

14. HTG control, black; HTG-1 , green ; HTG-2 , red

17. Results: 5、Pluripotencymarkers

19. Results: 6、High correlation : Genome-wide

22. 99 X-linked genes were overexpressed and 4 Y-linked genes were lost in haploid ES cells

23. Results: 7、 Differentiation potential :Chimaeras

28. Results: 8、Utility : Genetic screening

29. 1、6-TG（2氨基6巯嘌呤）2、Spilinkerette PCR

32. Thank you！