Ontogeny of embryogenic aggregates in suspension culture of diploid watermelon [Citrullus lanatus (Thunb.)]

Cell suspension culture from leaf-derived callus of diploid watermelon [Citrullus lanatus (Thunb.)] was established. The callus obtained on agar-gelled Murashige and Skoog (MS) medium containing 2.5 mgl-1 2,4-dichlorophenoxyacetic acid (2,4-D) was used for suspension culture. The growth of cell in the suspension culture was the highest in liquid MS medium containing same concentration (2.5 mgl-1) of 2,4-D as used for callus induction. Cells in suspension culture underwent division as result both free cells and cell aggregates were formed.

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Ontogeny of embryogenic aggregates in suspension culture of diploid watermelon [Citrullus lanatus (Thunb.)]

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