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Ligation Chart

Ligation Chart. C0261 (24A) Cut: S & P Primer: pSB1( A2 /A3/AK3). I13504 (12D) Cut: X & P Primer: I13504. F2620 (6B) Cut: S & P Primer: pSB1( A2 /A3/AK3). C0261 + I13504 Cut: X & P Primer: I13504. Riboswitch Cut: Pre-cut Primer: Riboswitch. C0261 + I13504 Cut: E & X

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Ligation Chart

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  1. Ligation Chart C0261 (24A) Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 (12D) Cut: X & P Primer: I13504 F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) C0261 + I13504 Cut: X & P Primer: I13504 Riboswitch Cut: Pre-cut Primer: Riboswitch C0261 + I13504 Cut: E & X Primer: pSB1A2 Primer (Reverse) F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) RS + C0261 + I13504 Cut: X & P Primer: I13504 Lox Sites Cut: UNKNOWN Primer: UNKNOWN C0261 + I13504 Cut: UNKNOWN Primer:UNKNOWN F2620 (6B) Cut: S & P Primer: pSB1(A2/A3/AK3) LOX + C0261 + I13504 Cut: X & P Primer: I13504 J37019 Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 Cut: X & P Primer: I13504 B0034 (3O) Cut: S & P Primer: pSB1(A2/A3/AK3) C0062 (7A) Cut: X & P Primer: C0062 2 R0062 (9G) Cut: S & P Primer: pSB1(A2/A3/AK3) B0034 + C0062 Cut: X & P Primer: C0062 2 I13033 (2H) Cut: S & P Primer: pSB1(A2/A3/AK3) I13504 (12D) Cut: X & P Primer: I13504 I13033 + I13504 Cut: X & P Primer: I13504 J37019 Cut: S & P Primer: pSB1(A2/A3/AK3) B0034 (3O) Cut: S & P Primer: pSB1(A2/A3/AK3) J37024 Cut: X & P Primer: UNKNOWN J37023 Cut: S & E Primer: UNKNOWN B0015 (1I) Cut: X & E Primer: UNKNOWN J04500 (16P) Cut: S & P Primer: pSB1(A2/A3/AK3) J37024 Cut: X & P Primer: UNKNOWN J37025 Cut: X &P Primer: J37024A J37019 Cut: S& P Primer: pSB1(A2/A3/AK3)

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