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E. Coli What's in your water?

E. Coli What's in your water?. Have you ever wondered what is in your water?. Is Spring or Stream Water more Contaminated with E. Coli?. Research. According to the USDA and the EPA, agricultural run-off can greatly contaminate water sources E. coli from animal dung. Springs vs. Streams.

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E. Coli What's in your water?

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  1. E. Coli What's in your water?

  2. Have you ever wondered whatis in your water?

  3. Is Spring or Stream Water more Contaminatedwith E. Coli?

  4. Research • According to the USDA and the EPA, agricultural run-off can greatly contaminate water sources • E. coli from animal dung

  5. Springs vs. Streams • Springs are underground streams. • Springs are naturally filtered. • Streams are shallow rivers. • Streams are exposed to contaminants

  6. Hypothesis Stream water will have the higher E. coli levels than spring water.

  7. Experimental Procedure- prepare the sample 1. Collect water samples in sterilized 25 mL glass jars 2. Add 5 mL of sampled water to bottle of nutrients and shake for5 seconds

  8. Materials For this experiment you would need. • Incubator • Petri dishes and corresponding supplies • Eye droppers • Water samples • Rubber gloves • Counting

  9. Experimental Procedure- growing the bacteria 3. Place the sample ina Petri dish after 3 minutes the solution gels. Invert dishes 5. The Petri dishes were incubated for 3 days at 23-26 C 4. Colonies are counted and results are graphed 5 Repeat steps 2 through 4 for all samples

  10. Incubation • Samples are incubated • Maintain a constant temperature of twenty-three(23) to twenty-six(26) degrees Celsius • All samples were isolates to a sterile surface to control cross contamination

  11. Experimental Procedure Counting • Count E.coli in each Petri dish -use a grid over the Petri dish and count the colonies - to ensure accurate data repeat counts

  12. Control Group • Distilled water- most impurities removed • Zero E. Coli should be present

  13. CONTROL

  14. STREAMS

  15. SPRINGS

  16. Average Coliform Count in 5mL sample

  17. Conclusion The data does not support my hypothesis that stream water has more E. coli than spring water. Due to the individuality of streams and springs.

  18. Further Improvements to test streams and their corresponding feeder springs to determine the contamination levels from the source and down-stream. the source of the contamination along the waterway.

  19. Abstract • Is Spring or Stream Water more Contaminatedwith E. Coli? • Stream water will have the higher E. coli levels than spring water. • The data supports my hypothesis that stream water has more E. coli than spring water.

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