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Journal Club 2: Science in Medicine

Journal Club 2: Science in Medicine. 5/10/12. Overview. www.dundeeresearch.wordpress.com Basic science papers vs medical papers Is basic science research important to clinicians and should we keep up with it? BACE1 paper. The importance of ‘basic’ sciences.

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Journal Club 2: Science in Medicine

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  1. Journal Club 2: Science in Medicine 5/10/12

  2. Overview • www.dundeeresearch.wordpress.com • Basic science papers vs medical papers • Is basic science research important to clinicians and should we keep up with it? • BACE1 paper

  3. The importance of ‘basic’ sciences • Foundation for most of our knowledge about how the body works, and all of our knowledge about how drugs work. • 1,500,000 scientific papers a year. • The scientist vs the clinician

  4. What to read? • To read or not to read? • Area of interest • Searching • Medline vs Google • Choice of Journal • Abstracts & Methods • Medical vs Scientific

  5. How to read it? • http://www.realscience.org.uk/newnews-and-resources-archive.html • Title, Abstract and Pictures • Read through once • Particularly Intro and Discussion • Analysis • Hypothesis -> Experiments -> Answer • Assumptions, Controls and the Whole Truth • Bigger Picture

  6. Reduction in BACE1 decreases body weight, protects against diet-induced obesity and enhances insulin sensitivity in mice MeakinP, et al. Biochem J. 2012 January 1; 441(Pt 1): 285–296.

  7. Background • Alzheimer’s and type II diabetes • BACE1 and KO mouse models

  8. Methods • Mouse models • WT vs KO • NC vs HFD • Cell models (Mouse myoblast cells) • BACE1 vs control plasmid transfection • BACE1 inhibitor treatments • Western Blotting and Taqman • Antibody and gene choices

  9. Summary of results • BACE1 KO mice • Reduced weight gain; insulin sensitive and leptin sensitive (reduced leptin and insulin levels) • Increased UCP expression • Cells treated with inhibitor • Increased insulin sensitivity and glucose uptake

  10. Discussion • Is BACE1 inhibition a novel drug target for diabetes/obesity? • Is this important to clinicians to know? • If yes, what are we still needing to know? • In short, everything.

  11. Scientific Research • It’s not all horrendous • Some of it is • It’s important... to scientists • It’s interesting to clinicians- but may become important

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