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CMK-T3 (n=2)

CMK-T12 (n=2). TP53 exon 6-148bp conventional PCR. TP53 exon 6-148bp TT- full -COLD PCR. CMK-T12 (n=2). CMK-T3 (n=2). CMK-T3 (n=2). CMK-T5 (n=2). CMK-T5 (n=2). wt (n=3). wt (n=3). Δ Fluorescence. TP53 exon 8-115bp conventional PCR. TP53 exon 8-115bp TT- full -COLD PCR.

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CMK-T3 (n=2)

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  1. CMK-T12 (n=2) TP53 exon 6-148bp conventional PCR TP53 exon 6-148bp TT-full-COLD PCR CMK-T12 (n=2) CMK-T3 (n=2) CMK-T3 (n=2) CMK-T5 (n=2) CMK-T5 (n=2) wt (n=3) wt (n=3) Δ Fluorescence TP53 exon 8-115bp conventional PCR TP53 exon 8-115bp TT-full-COLD PCR CT10 (n=2) CT10 (n=2) CMK-T7 (n=2) CMK-T7 (n=2) CT5 (n=2) CT20 (n=2) CT20 (n=2) CT5 (n=2) wt (n=3) wt (n=3) Temperature ºC Supplementary Figure 3. TT-full-COLD PCR-HRM and conventional-HRM analysis of clinical samples for high-, mid- and low abundance mutations present in exons 6 and 8 of TP53 gene (n= number of replicates).

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