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ADSORPTION OF FECAL COLIFORMS, ESCHERICHIA COLI IN DIFFERENT SOILS IN SARAWAK

ADSORPTION OF FECAL COLIFORMS, ESCHERICHIA COLI IN DIFFERENT SOILS IN SARAWAK. Ling Teck Yee, Goh Soon Hian and Kasing Apun Faculty of Resource Science & Technology University Malaysia Sarawak 94300 Kota Samarahan. INTRODUCTION. animal waste is a potential source of bacteria pathogens

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ADSORPTION OF FECAL COLIFORMS, ESCHERICHIA COLI IN DIFFERENT SOILS IN SARAWAK

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  1. ADSORPTION OF FECAL COLIFORMS, ESCHERICHIA COLI IN DIFFERENT SOILS IN SARAWAK Ling Teck Yee, Goh Soon Hian and Kasing Apun Faculty of Resource Science & Technology University Malaysia Sarawak 94300 Kota Samarahan

  2. INTRODUCTION • animal waste is a potential source of bacteria pathogens • microbial contamination occurs through animal wastewater discharge & lagoon effluent • adsorption of E. coli in soil depends on organism surface, soil particles & medium • microbial adsorption in soil increased with clay content & organic matter

  3. INTRODUCTION • microbial adsorption in soil increased with clay content (Ling et al., 2002; Weaver et al., 1978) &organic matter (Marshall, 1971) • Both solid waste and lagoon effluent application on land are viable options for animal waste management • soils that are not capable of adsorbing fecal bacteria can be avoided as waste disposal sites

  4. OBJECTIVES • to determine the adsorption kinetics of E. coli in a soil‑water system • to compare the adsorption of E. coli in three different types of soils

  5. MATERIALS & METHODS Soils Collection & Analysis • 3 different soils collected around Kuching • particle size analysis by Pipette method, Soil pH by pH meter, total organic matter by Loss-on-ignition method Bacteria Isolation & Identification • E. coli isolated from fresh farm wastewater • Gram test & API 20E diagnostic kit

  6. MATERIALS & METHODS Adsorption Kinetics • 6 ml of adjusted initial bacteria concentration (106 cfu/ml) was added to 6 g of soil • sampling at 0, 5, 15, 30, 60 & 120 minutes Batch Adsorption • using different initial bacteria concentrations from 102 to 1011 cfu/ml

  7. Table 1. Physical & chemical characteristics of the 3 different soils used in the study

  8. RESULTS & DISCUSSION • sorption equilibrium of E. coli between water & soil achieved immediately in 2 steps • percent sorption increased with initial bacteria concentrations at low bacteria concentrations • bacteria uptake over 99% at high bacteria concentrations • soils with higher clay & organic matter has significantly higher adsorption capacity

  9. Figure 1. E. coli depletion from 3 soils for 120 mins, initial bacteria concentrations of 4.50 x 106cfu/ml

  10. Figure 2. Percent of E. coli adsorbed with different initial concentrations in the 3 soils

  11. RESULTS & DISCUSSION • significantly higher bacterial adsorption than Commerce clay loam & San Angelo sandy clay loam (Ling et al., 2002; Weaver et al., 1978) • Daniels (1980), optimum adsorption of bacterial to soil particles generally occurs in low pH conditions • Hattori (1970), bacteria-clay complex is more stable in acidic conditions

  12. CONCLUSION • sorption equilibrium of E. coli was immediate • high E. coli removal in soil systems • effectiveness of bacteria removal is dependent on bacteria concentration • soil with higher clay & organic matter content has higher capacity for adsorbing E. coli

  13. ACKNOWLEDGEMENT The authors are grateful to University Malaysia Sarawak for providing the research grant (No.249/01)

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