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Ch. 15 Gene Technologies & Human Applications

Ch. 15 Gene Technologies & Human Applications. Mrs. K. Johnson LCHS. Fingerprinting Activity. Listen to the directions. You will work in lab groups. Materials: 1 ink pad 1 piece of paper Analyze results, examine differences.

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Ch. 15 Gene Technologies & Human Applications

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  1. Ch. 15 Gene Technologies & Human Applications Mrs. K. Johnson LCHS

  2. Fingerprinting Activity • Listen to the directions. • You will work in lab groups. • Materials: 1 ink pad 1 piece of paper • Analyze results, examine differences.

  3. What is Genetic Engineering?(Write this down.) • Genetic engineering is the transfer of genes from one organism to another. • We can recombine pieces of DNA from different organisms. • WHY? • To cure diseases (still in progress) • To treat diseases • Food Crops • Livestock • Basic Research Tool

  4. Gene Splicing(Write this down.) • Restriction enzymes cut DNA at precise spots. • The restriction enzyme used most often is Endonuclease. • It cuts the desirable DNA chunk out so it can then be put into another organism.

  5. The jellyfish glow gene has become a powerful tool for studying molecules inside living cells.

  6. Gene Splicing Example:The Glowing Tobacco Plant • R.E. cuts the “glowing” gene from a firefly’s DNA. • R.E. cuts open the tobacco plant’s DNA and the “glowing” gene is placed inside. • Ligase enzyme is used to seal or glue the DNA back together. • End result: Recombinant DNA

  7. 52 % of cotton grown last year was genetically engineered with a bacteria gene to resist bugs.

  8. Glow in the Dark Mouse A glowing mouse that had the glowing jellyfish gene implanted.

  9. A cat in Korea was engineered to glow in the dark.How could this help with disease research? The cat’s skin glows.

  10. DNA Sequencing(Write this down!) • Use a single strand of DNA • Cut it into sequences using a restriction enzyme • Gel electrophoresis p. 356 Fig. 10

  11. A Genetically Engineered Tomato, so what’s the BIG DEAL?

  12. Just as you each had different fingerprints, you also have different DNA sequences, DNA fingerprints. • You will use those differences in the following activity.

  13. Online DNA Fingerprinting • Go to this website: http://www.pbs.org/wgbh/ nova/sheppard/analyze.html Follow the instructions!

  14. Turn in one half-sheet for your lab group • Answer: Who is the… Holographic Lollipop Licker?

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