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BamH I. EcoR I. LB. Hpt. P 35S. TEVL. Chimeric gene. NOS. GUS. RB. Vector pS1301 carrying chimeric gene.
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BamHI EcoRI LB Hpt P35S TEVL Chimeric gene NOS GUS RB Vector pS1301 carrying chimeric gene SupplementaryFigure 1. Schematic diagrams of the transformation construct. RB and LB, right and left T-DNA border; GUS, -glucuronidase gene; Hpt, hygromycin phosphotransferase gene; P35S, cauliflower mosaic virus 35S promoter; TEVL, 5‘-nontranslated region of the tobacco etch virus; NOS, nopaline synthase polyadenylation signal. Four chimeric genes, D52S, D56S, D57S, and D92S, were cloned into this vector.