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Selected Case Studies

Selected Case Studies. MIT 2006: Engineering bacteria to smell good. C. breweri. S. cerevisiae. ATF1 banana. BSMT wintergreen. Slides borrowed from the 2006 MIT Team. Engineering the scent of bacteria. What is the desired output?. Components of engineered cell.

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Selected Case Studies

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  1. Selected Case Studies

  2. MIT 2006: Engineering bacteria to smell good C. breweri S. cerevisiae ATF1 banana BSMT wintergreen Slides borrowed from the 2006 MIT Team

  3. Engineering the scent of bacteria What is the desired output?

  4. Components of engineered cell What is needed to create the desired output?

  5. Components of engineered cell What is needed to create the desired output?

  6. Protein Regulatory Part/ Promoter Protein Coding Part Ribosome Binding Site Terminator Creating devices with BioBricks

  7. Components of scent-producing devices

  8. Components of scent-producing devices

  9. Components of upstream devices

  10. How do you regulate when the scents are expressed?

  11. Goal: Sense the growth state of culture

  12. Regulating the timing of expression • osmY: active in stationary phase & under high osmotic pressure conditions osmY osmY + inverter

  13. Regulating the timing of expression

  14. Design principles • What is the desired output? What is needed to create this output? • What do you want to sense? • Use of constitutive promoters, inducible promoters, and inverters

  15. Berkeley 2006Bacterial Networks Need: To transfer DNA messages from one bacterial cell to another Means: Bacterial Conjugation Need: To specifically control who can read the DNA message Means: Riboregulation Some slides borrowed from the 2006 Berkeley Team

  16. Ribosome Binding Site Protein Coding Part Regulatory Part/ Promoter Terminator Protein

  17. DNA to RNA to Protein DNA Ribosome Binding Site RNA Ribosome Binding Site Protein

  18. Protein synthesis from RNA Ribosome RNA Ribosome Binding Site Protein

  19. Ribosome Protein Locked structure prevents ribosome binding RNA Ribosome Binding Site

  20. Protein RNA “Key” unlocks lock Ribosome RNA Ribosome Binding Site

  21. Goal: Create network of cell-cell communication

  22. RNA as a regulatory mechanism • Can activate some “riboswitches” with small molecules • Advantage: fast response - transcript is already made • Alternative method: block translation by adding antisense RNA to RBD

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