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Frédéric AMARAL

In vitro assessment of shampoos eye stinging potential using Transient Receptor Potential Vanilloid Type 1 (TRPV1). Frédéric AMARAL. L’Oréal Research and Innovation , Aulnay, France. Presentation Outline. Role of TRPV1 in nociception Objectives of the study / experimental design

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Frédéric AMARAL

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  1. In vitro assessment of shampoos eye stinging potential using Transient Receptor Potential Vanilloid Type 1 (TRPV1) Frédéric AMARAL L’Oréal Research and Innovation , Aulnay, France

  2. Presentation Outline • Role of TRPV1 in nociception • Objectives of the study/experimental design • Results • Conclusions and nextsteps

  3. Role of TRPV1 in nociception (1) • Receptor type: calcium permeable ion channelbelonging to the TRP (TransientReceptorPotential) family • Location: C-fibers, CNS but also in skin, cornea and mucosal tissue • Activation: heat, acidic conditions, Capsaicin (CP) • calcium flux fromextracellular sources • Release of neurotransmiters, neuropeptides • induction of pain, burning, pruritus

  4. Role of TRPV1 in nociception (2) Need for an in vitro assay for identifying the stingingpotential of personal care products (ingredients and formulae)

  5. Objectives of the Study/experimental design • To demonstrate the PoCwith the « Nociocular » in vitro assay (based on SH-SY5Y overexpressing TRPV1) • Collaboration with the University of Stockholm (A Forsby) • Read out of the assay: calcium influx (fura2-AM) • Test articles used: • TRPV1 agonist: capsaicine (CP) for sensitivity • TRPV1 antagonist: capsazepine (CPZ) for specificity • A selection of 10 codedshampoos (adults and children/baby)

  6. Results: in vitro assay data • 3 categories of profiles have been identified Case A Case B Case C • induction of Ca2+ flux • No impact of CPZ • TRPV1-mediatedeffect? No induction of Ca2+ flux • induction of Ca2+ flux • Decreasedresponsewith CPZ • TRPV1-mediatedeffect

  7. Results: in vitro/in vivo correlation v v • Good correlation, especially for extreme classes • Good trend for the moderate class except for formula G

  8. Conclusions • We have demontratedthat: • The « Nociocular » assaycouldbeused to evaluateeyestingingpersonal care products • The assaywas applicable to complex water-soluble formula • For someformulae, the responsewas TRPV1-mediated • The in vitro responsescorrelatedwellwithclinical data • Nextsteps: • To evaluate a more diverse set of formulae and ingredients • To confirm the robustness of the assay • To refine the discomfort classes proposed • To develop a model for non water-soluble formulae • To sick for additionalassays to get a betterunderstanding of the responsesobserved

  9. Acknowledgments • L’OREAL • Linda Bourouf • Reine Note • Thomas Delanne • Gladys Ouédraogo • Sophie Loisel-Joubert • José Cotovio • Jean-Roch Meunier • University of Stockholm • HanegraafMaaike • Anna Forsby

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