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Lecture 4: Statistical Mechanics of Gene Regulation

Lecture 4: Statistical Mechanics of Gene Regulation. Rob Phillips California Institute of Technology. (Beautiful work of David Goodsell). The Single Molecule Census Continued: Gene Expression . Lac Repressor. RNAP+DNA+mRNA. Products of the Lac Operon. CRP and DNA.

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Lecture 4: Statistical Mechanics of Gene Regulation

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  1. Lecture 4: Statistical Mechanics of Gene Regulation Rob Phillips California Institute of Technology (Beautiful work of David Goodsell)

  2. The Single Molecule Census Continued: Gene Expression Lac Repressor RNAP+DNA+mRNA Products of the Lac Operon CRP and DNA (Beautiful work of David Goodsell)

  3. All Together Now: The Lac Operon Our goal: mathematize the story told by all of these cartoons. In particular, compute the probability of RNAP binding as a function of concentration of all the other molecular actors.

  4. Measure the Concentration of the Gene Product: Fluorescence (Elowitz and Leibler)

  5. Repression in the Lac Operon: the Role of Looping (Elowitz et al.)

  6. Beta-Galactosidase Activity (Dove et al.) (Lawrence et al.) Ian B. Dodd et al. Genes Dev. 2004; 18: 344-354

  7. Measuring the mRNA Content of a Cell (Yeast microarray data – Brown Group at Stanford)

  8. 3. intensifier 5. ccd detector 350,000 pixels 1. halogen tungsten lamp 2b. emission filters 2a. excitation flters 4. sample plate www.biorad.com Real-Time PCR

  9. Measuring Promoter Occupancy:ChIP http://www.vincibiochem.it/ChIP-IT.htm

  10. Repression in the Lac Operon: The Experimental Backdrop (Muller et al.)

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