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Biofabricator using light as the trigger

Imperial College 2008 iGEM Team http:// 2008.igem.org/Team:Imperial_College/Team Best Manufacturing Project Best New BioBrick Part, Natural. Biofabricator using light as the trigger. Overview. http:// 2008.igem.org/Team:Imperial_College Mechanism Light Sensing Motility Control

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Biofabricator using light as the trigger

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  1. Imperial College 2008 iGEM Teamhttp://2008.igem.org/Team:Imperial_College/Team Best Manufacturing Project Best New BioBrick Part, Natural Biofabricatorusing light as the trigger

  2. Overview • http://2008.igem.org/Team:Imperial_College • Mechanism • Light Sensing • Motility Control • Biomaterial Production • Process • Design • Modeling & Testing

  3. Background • Why Bacillus subtilis? • Single Membrane • High Motility

  4. Machanism • Integration • antibiotic resistance cassette • Light Sensing • Blue light sensitive • Motility Control • The Molecular Clutch • Biomaterial Production • Through the Membrane

  5. Modeling & Testing(1/3) • Growth Curve • Lag Phase • Exponential Phase • Stationary Phase

  6. Modeling & Testing(1/3)Cont. • Growth Curve • Result

  7. Modeling & Testing(2/3) • Constitutive Gene Expression

  8. Modeling & Testing(3/3) • Motility

  9. Modeling & Testing(3/3)Cont. • Motility

  10. Application • BioCouture

  11. Application (Cont.) • Medication • Elastin • Cartilage rebuild

  12. Reference • http://2008.igem.org/ • Alon U (2006) An Introduction to Systems Biology: design principles of biological circuits

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