1 / 2

Day 1, 23.9.2014 Download APE.exe and familiarize

Day 1, 23.9.2014 Download APE.exe and familiarize Look for Trypanosoma cruzi hexokinase in GenBank or in the TriTryp database

odette
Download Presentation

Day 1, 23.9.2014 Download APE.exe and familiarize

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Day 1, 23.9.2014 • Download APE.exe and familiarize • Look for Trypanosomacruzihexokinase in GenBankor in theTriTryp database • synthesizeforwardand reverse oligonucleotides to amplifythewhole gene, insertingsuitablerestriction sites in the 5´and 3´ ends, start with BamH1 (5´) and Xho1 (3´) sites, but do not include thestopcodon.

  2. Rest in peace *16.9.1928 + 24.9.2014 • Day 2, 25.9. 2014 • Lookupthesequence for eGFPandsynthesizeoligoswith a 5´ Sal1 site and a 3´ Xho1 site • Include a 3x Ala ou Gly – linker in the 5´ oligo • Fuse in APE theamplifiedfragment to theTcHexokinasefromtheotherday

More Related