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Katharina Giebel

The holy grail of footrot in sheep: Persistence of Dichelobacter nodosus , the causal agent of footrot in sheep. Katharina Giebel. Background. Ovine footrot (OFR) Economically important Negative effects on animal welfare and productivity Farmer estimated prevalence 10.4 % (UK). Background.

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Katharina Giebel

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  1. The holy grail of footrot in sheep: Persistence of Dichelobacter nodosus, the causal agent of footrot in sheep • Katharina Giebel

  2. Background Ovine footrot (OFR) • Economically important • Negative effects on animal welfare and productivity • Farmer estimated prevalence 10.4 % (UK)

  3. Background • Dichelobacter nodosus: causative infectious agent • Gram negative anaerobic bacterium

  4. Pathogenesis Accepted pathogenesis: 1. Primary infection with Fusobacterium necrophorum 2. D. nodosus invades damaged tissue and becomes established in the foot

  5. Transmission of D. nodosus

  6. Importance of the environment • Important factor influencing disease transmission • Environmental conditions favourable for Dn • Disease reappears after periods of non transmission • ? Must persist!

  7. Evidence for persistence of D. nodosus • Old evidence

  8. Evidence for persistence of D. nodosus • New evidence: • Cross-sectional and longitudinal studies

  9. Aims • Investigate persistence of D.nodosus in the environment by identifying possible sites of persistence and estimating its duration • Investigate associations between D. nodosus presence/persistence and levels of disease in a flock over time

  10. Methods

  11. Methods

  12. Methods

  13. Progress so far…. DNA extraction from soil samples1: Soil from pasture (SP), 2: soil from hoof print (SH) 1 2

  14. Progress so far…. PCR from soil samples1: Soil from pasture (SP), 2: soil from hoof print (SH) 2 1

  15. Progress so far…. PCR from soil samples (Different dilutions of DNA) 1: Soil from pasture (SP), 2: soil from hoof print (SH) 1 2

  16. In the next few weeks…

  17. Some challenges

  18. Thank you for listening!

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