Download
1 / 28
350 likes | 830 Views
PTT 202: ORGANIC CHEMISTRY FOR BIOTECHNOLOGY. NUCLEIC ACIDS. PREPARED BY: NOR HELYA IMAN KAMALUDIN helya@unimap.edu.my. Introduction to Nucleic Acids. Isolation and Purification of Nucleic Acids. Stages in Isolation and Purification of Nucleic Acids.
E N D
PTT 202: ORGANIC CHEMISTRY FOR BIOTECHNOLOGY NUCLEIC ACIDS PREPARED BY: NOR HELYA IMAN KAMALUDIN helya@unimap.edu.my
Isolation and Purification of Nucleic Acids Stages in Isolation and Purification of Nucleic Acids
Isolation of total cellular DNA Figure 1; Caesium chloride density gradient centrifugation for (a) the separation of DNA from RNA and protein and (b) the separation of linear DNA and supercoiled DNA.
Isolation of RNA Proteinase K method
Isolation of RNA • Guanidine thiocyanate method
Spectrophotometric methods Figure 2: Melting temperature for DNA
The Maxam and Gillbert Method Figure 3: Aliquots of DNA fragment
The Maxam and Gillbert Method Figure 4: The Maxam and Gillbert method of DNA sequencing.
The Dideoxy Method Figure 5: The dideoxy (chain termination) method of DNA sequencing
