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All Done with Mirrors: Smart Substrates for 3D Views

All Done with Mirrors: Smart Substrates for 3D Views. Dr. J. Alexander Liddle Dr. Matthew McMahon, Dr. Andrew Berglund, Dr. Peter Carmichael, Dr. Jabez McClelland. Benefits/Advantages. Microfabricated substrates can be combined with most other optical microscopy techniques Simple

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All Done with Mirrors: Smart Substrates for 3D Views

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  1. All Done with Mirrors: Smart Substrates for 3D Views Dr. J. Alexander Liddle Dr. Matthew McMahon, Dr. Andrew Berglund, Dr. Peter Carmichael, Dr. Jabez McClelland

  2. Benefits/Advantages • Microfabricated substrates can be combined with most other optical microscopy techniques • Simple • Easy to make • Add lots of information • Function: Resolve ambiguity • Same positions when viewed from above, but different heights • Application: Put cell inside sample well • Rapid screening of cell height • Modest-resolution, high-throughput 3D information

  3. What Motivated the Research? Cui, Björk, Liddle, Sönnichsen, Boussert & Alivisatos Nano Lett.2004, 1093 Solution-Based Nanoparticle Assembly

  4. 3D Imaging in Optical Microscope • Goal: Monitor/Measure Dynamics by Seeing in 3D • Track assembling particles in as close to real-time as possible • The smaller they are, the faster they move • How?

  5. 2D Track from 2D Images • 2D tracks easily constructed from 2D video • Special software needed to get accurate particle positions • Some codes available free

  6. The ideal would be… Oh well…hmmm….

  7. Orthogonal Tracking Microscopy Reduce the problem of tracking in 3Dto the problem of tracking in 2D twice MICROFABRICATED SAMPLE CHAMBER WITH INTEGRATED REFLECTIVE OPTICS Si

  8. Software Enhances Technology • New algorithms developed that are fast and can handle complicated shapes Difficult Easy

  9. Demonstration • All-silicon substrate • Squares defined by photolithography • Anisotropic etch of Si by KOH: 54.7° sidewalls • Pyramidal indentations Movie: 190 nm fluorescent polystyrene sphere in water

  10. Future Development • Hardware: • Integrate more complex optical elements • Better mirrors (i.e. gold) • Filters • Polarizers • Diffractive elements • Software • Explore asymmetry • Asymmetric objects: rotational motion • Asymmetric radiation patterns

  11. Summary • Microfabricated substrates can be combined with most other optical microscopy techniques • Resolve ambiguity • Same positions when viewed from above but different heights • Put cell inside sample well • Rapid screening of cell height • Modest-resolution, high-throughput 3D information • A patent application covering the concept of using microfabricated optics to permit imaging in 3D is pending.

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