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AFFYMETRIX GENECHIP ARRAY STATION. By. Pierce Chuang, Vishu Rajamani, Jeremy Rasiah. Overview. Introduction to DNA/RNA Sequencing Techniques Affymetrix GeneChip Station GeneChip Station Architecture Manufacturing Process Questions. Introduction to DNA/RNA. Introduction DNA/RNA (cont’d).
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AFFYMETRIX GENECHIP ARRAY STATION By Pierce Chuang, Vishu Rajamani, Jeremy Rasiah
Overview • Introduction to DNA/RNA • Sequencing Techniques • Affymetrix GeneChip Station • GeneChip Station Architecture • Manufacturing Process • Questions
DNA Sequencing Techniques • Chain-terminator method (Sanger) • Dideoxynuclotides triphosphates (ddNTPs) as DNA chain terminators • Sequence roughly 550,000 bases in a day • Requires thirty years of uninterrupted operation to sequence all nucleotides in the human genome
DNA Sequencing Techniques • Sequencing By Hybridization (SBH) • Ex, target DNA sequence, AGCCTAGCTGAA Affymetrix Microarray Specification
GeneChip Array source: http://bach2.biosci.arizona.edu/
Results source: http://www.med.upenn.edu/
Detector Architecture source: http://www.affymetrix.com/
GeneChip Array source: http://bach2.biosci.arizona.edu/
Array Manufacturing Process • 2: substrates, SiO2, Si, GaAs, or Glass. • 6: Linker Molecules. Ex, Aryl Acetylene • 8: Mask, for photolithographic process • 10: Activated Linker Molecules
Array Manufacturing Process • This process is repeated several times to produce longer probes. • Full length probes are usually 25 nucleotides long