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Enzymes that make and consume Nitrous Oxide

DJ Richardson. INCREASE IN ATMOSPHERIC N2OEstimated that today 76% arises from agricultural soils . . . Haber-Bosch process for fixation of atmospheric Nitrogen. Centre forMetalloproteinSpectroscopy

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Enzymes that make and consume Nitrous Oxide

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    1. Enzymes that make and consume Nitrous Oxide

    3. How Do Soil Bacteria Respond to an Unlimited Supply of Fixed Nitrogen?

    6. The Enzyme that Produces N2O is Nitric Oxide Reductase

    7. Heme Iron Assists Nitric Oxide Reductase Transform NO to N2O by……

    8. Our work involves the isolation and characterisation of Bacterial NOR

    9. Three Hemes form a Nanowire that Conduct Electrons to the Site of N2O Formation

    10. We Have Identified a Channel that Moves Protons to the Site of N2O Formation

    11. N2O Formation is Controlled by the Rate at Which Protons are Delivered??

    12. Why do we Need to Understand the Rate of N2O Production?

    15. Different colours of N2OR

    18. Nitrous oxide reductase

    19. Catalytic redox-states of CuZ

    21. Where next? To describe the catalytic process in molecular terms To understand the interaction between NOR and N2OR To understand biosynthesis of the catalyst CuZ To understand the genetic regulation of the enzyme - e.g. dependence on copper

    22. Bacterial species that can Reduce N20 to N2 as an autonomous respiratory process Reduce Nitrate to N2O Reduce Nitrate to N2 producing N2O to varying degrees

    23. Regulation of Denitrification

    26. NO Reductase

    27. N2O Reductase

    28. www.nitrousoxide.org

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